Microbes make the meal: oligolectic bees require microbes within their host pollen to thrive
Microbes make the meal: oligolectic bees require microbes within their host pollen to thrive
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DOI：
10.1111/een.12926
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发表时间：
2020-08-12
影响因子：
2.2
通讯作者：
Steffan, Shawn A.
中科院分区：
文献类型：
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作者：
Dharampal, Prarthana S.;Hetherington, Matthew C.;Steffan, Shawn A.
1.对于专门选择花粉类型（寡聚体）的独居蜜蜂，幼虫发育取决于来自适当宿主植物的饲料花粉的可用性和其中存在的天然微生物群。虽然获得宿主花粉可能对寡聚蜜蜂的发育至关重要，但花粉微生物群对其繁殖成功的贡献程度尚不清楚。2.为了研究这一问题，我们采用了饲料控制实验，在体外条件下培育了寡肢壁蜂（Osmia ribifloris）的幼虫。幼虫饲养的寄主花粉提供的母亲或非寄主花粉收集的蜜蜂，在存在或不存在相应的花粉相关的微生物群。我们评估了对幼虫适合度组成部分的影响：发育时间，生物量和存活率。3.我们的研究结果揭示了花粉类型和花粉相关微生物之间的显着相互作用。微生物对幼虫生产性能的相对影响远大于花粉类型。宿主花粉基质产生了最适合的幼虫，但只有当与其天然存在的微生物群的完整补充相结合时。相反，没有微生物的寄主花粉导致适合度组分显著下降。幼虫消费非宿主花粉表现出中等健身，无论是否存在微生物或不。4.这些发现意味着与母体提供的宿主花粉相关的微生物群在幼虫营养和存活中起着关键作用。特别是对于寡聚体，在质量较差的宿主花粉上发育的能力可能来自与其微生物外共生体的持续共生，而不是单独的花粉类型的生化特征。
1. For solitary bees that specialise on select pollen types (oligoleges), larval development depends on the availability of forage pollen from appropriate host plants and the naturally occurring microbiota present therein. While access to host pollen may be critical for the development of oligolectic bees, the extent to which pollen microbiota contribute to their brood success is unknown. 2. To investigate, we used a diet manipulation experiment to rear larvae of the oligolege,Osmia ribifloris, underin-vitroconditions. Larvae were reared either on host pollen provisioned by their mother or on non-host pollen collected by honey bees, in the presence or absence of the respective pollen-associated microbiota. We assessed impacts on components of larval fitness: developmental time, biomass, and survivorship. 3. Our results revealed a significant interaction between pollen type and pollen-associated microbes. The relative effect of microbes on larval performance was substantially greater than that of pollen type. Host pollen substrate produced the fittest larvae but only when combined with its full complement of naturally occurring microbiota. In contrast, host pollen without microbes resulted in a marked decline in fitness components. Larvae consuming non-host pollen showed intermediate fitness, regardless of whether microbes were present or not. 4. These findings imply that the microbiota associated with maternally provisioned host pollen perform critical functions in larval nutrition and survival. For oligoleges in particular, the ability to develop on poorer quality host pollen likely derives from this sustained symbiosis with their microbial exosymbionts, rather than the biochemical characteristics of pollen type alone.