Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
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DOI：
10.1038/s41598-019-55083-0
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发表时间：
2019-12-06
影响因子：
4.6
通讯作者：
Christian, Hannig
中科院分区：
文献类型：
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作者：
Jasmin, Kirsch;Matthias, Hannig;Christian, Hannig
本临床实验研究旨在检验纯实验氟化物溶液和氯化亚锡对原位条件下初始口腔生物粘附的影响。在牛牙釉质板上形成表膜 1 分钟后，12 名受试者用 8 ml 氟化物浓度为 500 ppm 的氟化物测试溶液（NaF、Na2PO3F、AmF、SnF2）冲洗 1 分钟。此外，不使用溶液冲洗（对照）并使用 1563 ppm SnCl2 溶液冲洗 1 分钟。然后，使用 DAPI 和 ConA 以及 BacLight 方法进行荧光显微镜观察细菌粘附和葡聚糖形成（8 小时口腔暴露）。进行 TEM 来观察薄膜超微结构，并结合 EDX 来检测亚锡离子。含有纯 SnF2 和 SnCl2 的冲洗溶液显着减少了初始细菌定植 (DAPI)。而NaF和Na2PO3F与对照相比没有表现出显着效果。 AmF、SnF2和SnCl2之间没有显着差异。所有测试的实验溶液均未显示出对葡聚糖形成的减少作用。用含锡溶液冲洗导致薄膜超微结构发生相当大的变化。 SnF2 似乎是减少初始细菌原位定植最有效的氟化物类型。观察到的效果主要归因于亚锡离子的含量。
The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solutions (NaF, Na2PO3F, AmF, SnF2,) with 500 ppm fluoride concentration each for 1 min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl2 solution took place for 1 min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF2 and SnCl2 reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na2PO3F showed no significant effect compared to the control. There was no significant difference between AmF, SnF2 and SnCl2. All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF2 appears to be the most effective type of fluoride to reduce initial bacterial colonization in situ. The observed effects primarily have to be attributed to the stannous ions' content.