Sequential evolution of a symbiont inferred from the host:: Wolbachia and Drosophila simulans
Sequential evolution of a symbiont inferred from the host:: Wolbachia and Drosophila simulans
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DOI：
10.1093/molbev/msh028
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发表时间：
2004-03-01
影响因子：
10.7
通讯作者：
Ballard, JWO
中科院分区：
文献类型：
--
作者：
Ballard, JWO
本研究的目的是通过预测共生体会在宿主上留下感染的特征来揭示模型共生体/宿主系统的病毒学。具体而言，来自33个国家和64个采样地点的1,442个果蝇模拟的全球样本被用来推断母系遗传的α-变形菌沃尔巴克氏体的地理分布。系统发育分析表明，4个Wolbachia菌株均感染了D.一次模拟。Wolbachia菌株和三个mtDNA单倍型群（D. simulans siI、siII和siIII）。最后，siI（来自另外7个品系的584 bp）和sill（来自383个品系的1,701 bp）内可变区的网络分析促进了详细的电泳讨论。SI/III的变异很小，单倍型群的分布受到限制。这些数据显示了如何通过结合来自共生体和宿主的数据来绘制感染历史。他们很少提到宿主的生物历史，因为mtDNA基因组是整个基因组的一个有偏见的代表。
This study aims to unravel the biogeography of a model symbiont/host system by exploiting the prediction that a symbiont will leave a signature of infection on the host. Specifically, a global sample of 1,442 Drosophila simulans from 33 countries and 64 sampling localities was employed to infer the phylogeography of the maternally inherited alpha-proteobacteria Wolbachia. Phylogenetic analyses, from three symbiont genes and 24 mtDNA genomes (excluding the A + T-rich region), showed that each of four Wolbachia strains infected D. simulans once. The global distribution and abundance of the Wolbachia strains and the three mtDNA haplogroups (D. simulans siI, siII and siIII) was then determined. Finally, network analyses of variable regions within siI (584 bp from seven additional lines) and sill (1,701 bp from 383 lines) facilitated a detailed biogeographic discussion. There is little variation in si/III and the haplogroup is restricted in its distribution. These data show how the history of an infection can be mapped by combining data from the symbiont and the host. They say little about the organismal history of the host because the mtDNA genome is a biased representation of the whole genome.