Proteomic analysis of secretion from human transplanted submandibular gland replacing lacrimal gland with severe keratoconjunctivitis sicca.
Proteomic analysis of secretion from human transplanted submandibular gland replacing lacrimal gland with severe keratoconjunctivitis sicca.
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DOI：
10.1016/j.bbapap.2012.01.011
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发表时间：
2012-04
期刊：
影响因子：
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通讯作者：
B. Song;Lei Zhang;Xiao-jing Liu;C. Ding;Li-Ling Wu;Y. Gan;G. Yu
中科院分区：
文献类型：
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作者：
B. Song;Lei Zhang;Xiao-jing Liu;C. Ding;Li-Ling Wu;Y. Gan;G. Yu
目的对重度重度重度角膜结膜炎患者移植或未移植颌下腺分泌物及正常眼泪液进行蛋白质组学分析。实验设计收集5例重度重度重度角膜结膜炎患者1年移植替代泪腺和未移植的颌下腺的分泌物，3例正常人的泪液。2-D电泳（2-DE），然后用质谱法鉴定蛋白质。Western blot分析证实蛋白表达。结果在移植颌下腺和泪液的唾液中分别鉴定出34种和11种不同的蛋白质。移植颌下腺的唾液几乎含有泪液中丰富的所有蛋白质。颌下腺移植唾液中所鉴定的蛋白主要具有免疫应答和抗菌功能。共有7种蛋白在移植和未移植的颌下腺唾液中表达差异。Western blot分析证实了短腭、肺和鼻上皮癌相关蛋白2和碳酸酐酶VI的上调。结论颌下腺移植后唾液中所鉴定的蛋白对眼部结构具有保护作用。这些发现有助于了解移植颌下腺的功能状态。
PurposeProteomic analysis of secretions from transplanted or non-transplanted submandibular glands in patients with severe keratoconjunctivitis sicca and tears from normal eyes.Experimental designSecretions from submandibular glands transplanted to replace lacrimal glands and non-transplanted submandibular glands were collected at 1year from 5 patients with severe keratoconjunctivitis sicca undergoing transplantation, and tears were collected from 3 normal subjects. 2-D electrophoresis (2-DE), then mass spectrometry was used to identify proteins. Western blot analysis was used to confirm protein expression.ResultsWe identified 34 and 11 distinct proteins in the saliva from transplanted submandibular glands and tears, respectively. The saliva from transplanted submandibular glands contained almost all the proteins abundant in tear fluid. The functions of identified proteins in the saliva from transplanted submandibular gland were mainly immune response and anti-bacterial. In total, 7 proteins showed differential expression between the saliva of transplanted and non-transplanted submandibular glands. The upregulation of short palate, lung and nasal epithelium carcinoma-associated protein 2 and carbonic anhydrase VI was confirmed by Western blot analysis.ConclusionsIdentified proteins in saliva from transplanted submandibular glands may protect ocular structures. These findings can help in understanding the functional status of transplanted submandibular glands.