Hyperbaric oxygen therapy can ameliorate the EMT phenomenon in keloid tissue.
Hyperbaric oxygen therapy can ameliorate the EMT phenomenon in keloid tissue.
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DOI：
10.1097/md.0000000000011529
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发表时间：
2018-07
期刊：
影响因子：
1.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Zhang M;Liu S;Guan E;Liu H;Dong X;Hao Y;Zhang X;Zhao P;Liu X;Pan S;Wang Y;Wang X;Liu Y
高压氧治疗（HBOT）已广泛应用于临床。在这项研究中，HBOT疗法被评估其改善瘢痕疙瘩组织上皮-间质转化（EMT）现象的能力。瘢痕疙瘩患者随机分为两组：瘢痕疙瘩患者（K组，9例）和接受HBOT治疗的瘢痕疙瘩患者（O组，9例）。另设皮肤正常组（S组，9例）作为对照。在HBOT和手术前，使用激光多普勒流量计测量O组患者瘢痕疙瘩血供。苏木精和伊红（H&E）染色观察形态学。免疫荧光染色和Western blot检测E-cadherin、ZO-1、vimentin、纤维连接蛋白、血管内皮生长因子（VEGF）、缺氧诱导因子(HIF)-1α的含量。采用实时定量聚合酶链反应（RT-qPCR）评价这些因子的mRNA表达水平。O组患者接受HBOT后瘢痕疙瘩血灌注明显减少。与K组比较，O组vimentin、vibronectin、VEGF、HIF-1α表达水平较低，E-cadherin、ZO-1表达水平显著升高。HBOT后E-cadherin和ZO-1 mRNA的表达也增加。瘢痕疙瘩患者接受HBOT后，EMT现象相关因子的表达水平明显逆转，提示HBOT可能是一种有效治疗瘢痕疙瘩患者EMT现象的方法。
Hyperbaric oxygen therapy (HBOT) has been widely used in the clinical setting. In this study, HBOT therapy was evaluated for its ability to ameliorate the epithelial-to-mesenchymal transition (EMT) phenomenon in keloid tissue. Keloid patients were randomly divided into two groups: keloid patients (K group, 9 patients) and keloid patients receiving HBOT (O group, 9 patients). A third group with normal skin (S group, 9 patients) was established for control. Before HBOT and surgery, a laser Doppler flowmeter was used to measure the keloid blood supply of patients in the O group. Hematoxylin and eosin (H&E) staining was used to observe morphology. E-cadherin, ZO-1, vimentin, fibronectin, vascular endothelial growth factor (VEGF), and hypoxia inducible factor (HIF)-1α were measured by immunofluorescence staining and Western blot analysis. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the mRNA expression level of these factors as well. In the O group, keloid blood perfusion was significantly reduced after patients received HBOT. Compared with the K group, lower expression levels of vimentin, vibronectin, VEGF, and HIF-1α were observed in the O group, whereas the expression of E-cadherin and ZO-1 was significantly higher. The mRNA expression of E-cadherin and ZO-1 was also increased after HBOT. The expression levels of factors related to the EMT phenomenon were significantly reversed in keloid patients after they received HBOT, indicating that HBOT may be an effective therapy against the EMT phenomenon in keloid patients.