Ephrin-As guide the formation of functional maps in the visual cortex
Ephrin-As guide the formation of functional maps in the visual cortex
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DOI：
10.1016/j.neuron.2005.10.026
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发表时间：
2005-11-23
期刊：
影响因子：
16.2
通讯作者：
Feldheim, DA
中科院分区：
文献类型：
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作者：
Cang, JH;Kaneko, M;Feldheim, DA
Ephrin-As及其受体EphAs在发育中的皮质中表达，在那里它们可以起组织丘脑输入的作用。在这里，我们映射的视觉皮层（V1）在小鼠缺乏ephrin-A2，-A3，和-A5功能，使用内在信号光学成像和微电极记录，和结构，通过解剖跟踪丘脑皮质投射。V1向内侧移位、旋转和压缩，其内部组织退化。表达肝配蛋白-A5异位子宫内电穿孔在外侧皮质转移地图的V1媒体，和表达内V1破坏其内部组织。这些研究结果表明，EphA/肝配蛋白-A在皮质中的梯度之间的相互作用引导地图的形成，但多余的肝配蛋白-A以外的因素是负责残留地图。结合早期对视网膜神经元地图的研究，目前的研究结果表明，相同的分子相互作用可能在视觉通路的连续阶段起作用，以组织地图。
Ephrin-As and their receptors, EphAs, are expressed in the developing cortex where they may act to organize thalamic inputs. Here, we map the visual cortex (V1) in mice deficient for ephrin-A2, -A3, and -A5 functionally, using intrinsic signal optical imaging and microelectrode recording, and structurally, by anatomical tracing of thalamocortical projections. V1 is shifted medially, rotated, and compressed and its internal organization is degraded. Expressing ephrin-A5 ectopically by in utero electroporation in the lateral cortex shifts the map of V1 medially, and expression within V1 disrupts its internal organization. These findings indicate that interactions between gradients of EphA/ephrin-A in the cortex guide map formation, but that factors other than redundant ephrin-As are responsible for the remnant map. Together with earlier work on the retinogeniculate map, the current findings show that the same molecular interactions may operate at successive stages of the visual pathway to organize maps.