EXPRESSION OF IGF-I AND INSULIN-RECEPTOR GENES IN THE RAT CENTRAL-NERVOUS-SYSTEM - A DEVELOPMENTAL, REGIONAL, AND CELLULAR ANALYSIS
EXPRESSION OF IGF-I AND INSULIN-RECEPTOR GENES IN THE RAT CENTRAL-NERVOUS-SYSTEM - A DEVELOPMENTAL, REGIONAL, AND CELLULAR ANALYSIS
复制标题
DOI：
10.1002/jnr.490280212
复制
发表时间：
1991-02-01
影响因子：
4.2
通讯作者：
VANOBBERGHEN, E
中科院分区：
文献类型：
--
作者：
BARONVANEVERCOOREN, A;OLICHONBERTHE, C;VANOBBERGHEN, E
使用溶液杂交测定和特异性寡核苷酸探针，我们研究了发育过程中大鼠中枢神经系统中的 IGF-I 和胰岛素受体 mRNA。 IGF-I受体的mRNA表达在胚胎第15天和第20天达到最大，胰岛素受体的mRNA表达在胚胎第20天和出生当天达到最大。 出生后，两种受体转录物的表达均下降，并在成人中达到最低水平。 在这些转录物表达量最大时（胚胎第20天），区域分析表明IGF-I受体转录物广泛分布在大脑中。 相反，胰岛素受体转录本仅限于与 IGF-I 受体转录本共表达的某些区域。 接下来我们分析了胚胎第 20 天的哪些细胞表达了这些受体转录本。 晚期胚胎神经元、星形胶质细胞和少突胶质细胞的新生儿祖细胞在培养较短时间后合成IGF-I和胰岛素受体mRNA。 然而，星形胶质细胞优先表达IGF-I受体转录本，而少突胶质细胞的年轻祖细胞则表达高水平的胰岛素受体转录本。 总的来说，我们的数据表明，在大鼠 CNS 发育过程中，IGF-I 和胰岛素受体的表达似乎具有阶段和细胞特异性。 观察到的两种受体表达之间的差异可能表明 IGF-I 和胰岛素及其受体在此期间具有特定但协调的作用。
Using a solution-hybridization assay and specific oli-gonucleotidic probes, we have studied IGF-I and insulin receptor mRNAs in the rat central nervous system during development. The expression of mRNAs was maximal at embryonic day 15 and 20 for IGF-I receptors, and at embryonic day 20 and the day of birth for insulin receptors. After birth, the expression of both receptor transcripts decreased and reached minimal levels in the adult. At the time at which these transcripts were maximally expressed (embryonic day 20), the regional analysis indicated that IGF-I receptor transcripts were widely distributed in the brain. In contrast, insulin receptor transcripts were restricted to certain areas in which they were coexpressed with the IGF-I receptor transcripts. We next analyzed which cells at embryonic day 20 expressed those receptor transcripts. Late embryonic neurons, astrocytes, and neonatal progenitors of oligodendrocytes synthesized both IGF-I and insulin receptor mRNAs after a short time in culture. However, astrocytes expressed preferentially IGF-I receptor transcripts, while young progenitors for oligodendrocytes expressed high levels of insulin receptor transcripts. As a whole, our data indicate that during rat CNS development expression of IGF-I and insulin receptors appears to be stage- and cell-specific. The differences observed between the expression of both receptors might point to a specific, but coordinative role of IGF-I and insulin and their receptors during that time.