Thymine-thymine dimer bypass by yeast DNA polymerase zeta
Thymine-thymine dimer bypass by yeast DNA polymerase zeta
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DOI：
10.1126/science.272.5268.1646
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发表时间：
1996-06-14
期刊：
影响因子：
56.9
通讯作者：
Hinkle, DC
中科院分区：
文献类型：
--
作者：
Nelson, JR;Lawrence, CW;Hinkle, DC
酿酒酵母的 REV3 和 REV7 基因是 DNA 损伤诱导诱变所必需的。 Rev3 和 Rev7 蛋白被证明可形成具有 DNA 聚合酶活性的复合物。这种聚合酶复制经过胸腺嘧啶-胸腺嘧啶顺式-顺式环丁烷二聚体，这种损伤通常会严重抑制复制，效率约为 10%。相比之下，酵母 DNA 聚合酶 a 的旁路复制效率不超过 1%。 Rev3-Rev7 复合物是要描述的第六种真核 DNA 聚合酶，因此称为 DNA 聚合酶 zeta。
The REV3 and REV7 genes of the yeast Saccharomyces cerevisiae are required for DNA damage-induced mutagenesis. The Rev3 and Rev7 proteins were shown to form a complex with DNA polymerase activity. This polymerase replicated past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally severely inhibits replication, with an efficiency of similar to 10 percent. In contrast, bypass replication efficiency with yeast DNA polymerase a was no more than 1 percent. The Rev3-Rev7 complex is the sixth eukaryotic DNA polymerase to be described, and is therefore called DNA polymerase zeta.