Mouse orthotopic models for bladder cancer research
Mouse orthotopic models for bladder cancer research
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DOI：
10.1111/j.1464-410x.2009.08577.x
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发表时间：
2009-11-01
影响因子：
4.5
通讯作者：
Larchian, William
中科院分区：
文献类型：
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作者：
Chan, Eddie;Patel, Amit;Larchian, William
膀胱癌是泌尿系统常见的恶性肿瘤。尽管有不同的治疗选择，尿路上皮癌治疗后复发和进展并不罕见。膀胱癌的新的治疗选择是迫切需要的。新疗法的临床前评估需要一种模仿人类肿瘤模型的动物肿瘤模型。迄今为止，已经描述了各种动物原位膀胱癌模型，但报道的肿瘤“摄取”率为30- 100%。建立可靠和可重复的动物模型仍然是一个持续的挑战。本文综述了用于尿路上皮癌研究的各种原位膀胱癌小鼠模型、移植方法和肿瘤发生率。最近在肿瘤模型的非侵入性小动物成像方面取得了重大进展。现在，研究人员可以通过直接和非侵入性地监测体内肿瘤生长以及测量小动物中广泛的肿瘤相关变量来研究所研究药物的作用。我们总结了最近的发展，在小动物成像肿瘤检测和定量。
Bladder cancer is a common malignancy in the urinary tract. Despite different therapeutic options, recurrence and progression of urothelial carcinoma after treatment is not uncommon. Novel therapeutic options of bladder cancer are urgently needed. The preclinical evaluation of new treatments requires an animal tumour model that mimics the human counterpart. To date, various animal orthotopic bladder cancer models have been described, but the reported rate of tumour 'take' is 30-100%. The establishment of reliable and reproducible animal models remains an ongoing challenge. We review different kinds of mouse models of orthotopic bladder cancer used in urothelial cancer studies, the methods of implantation, and the reported rate of tumour take. Significant progress has been made recently in noninvasive small animal-imaging in tumour models. It is now possible for researchers to investigate the effects of studied agents by monitoring of in vivo tumour growth directly and noninvasively, as well as measuring a wide range of tumour-related variables in small animals. We summarize the recent development in small-animal imaging for tumour detection and quantification.