Lifetime expression of stem cell markers in the uterine endometrium
Lifetime expression of stem cell markers in the uterine endometrium
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DOI：
10.1016/j.fertnstert.2003.07.015
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发表时间：
2004-02-01
影响因子：
6.7
通讯作者：
Kim, SK
中科院分区：
文献类型：
--
作者：
Cho, NH;Park, YK;Kim, SK
目的：鉴定胎儿至绝经后子宫内干细胞。设计：描述性对照研究。地点：三级医学中心。患者(S)：40例存档子宫内膜标本。干预(S)：c-kit/CD117、CD34、bcl2、Ki67免疫组织化学染色(S)：免疫组织化学(S)：免疫组织化学染色(S)：c-kit/CD117、CD34、bcl2、Ki67在基底层间质中一致表达。结论：通过对干细胞标志物c-kit、CD34和bcl2的免疫定位研究，发现干细胞从胎儿时期起就在子宫内膜中持续存在。这些细胞似乎主要位于基底层的基质中，不受激素调节，以避免周期性脱落。少数潜伏在基底层基质中的干细胞具有向间充质分化的潜能。(C)2004年，由美国生殖医学会提供。
Objective: To identify stem cells in uteri from the fetal to the postmenopausal period.Design: Descriptive, controlled study.Setting: Tertiary academic medical center.Patient(s): Forty archived uterine endometrial samples.Intervention(s): Immunohistochemistry with c-kit/CD117, CD34, bcl-2, and Ki67.Main Outcome Measure(s): Immunostaining.Result(s): C-kit/CD117, CD34, bcl-2, and Ki67 were consistently expressed in the stroma of the basalis layer. Bcl-2 was restricted to the fetal uterine endometrium.Conclusion(s): By studying the immunolocalization of stem cell markers (i.e., c-kit, CD34, and bcl-2), we found that stem cells are present continuously in the uterine endometrium from the fetal period. The cells seem to be located mainly in the stroma of the basalis and to be independent of hormonal regulation to avoid cyclic shedding. A small number of latent stem cells dormant in the stroma of the basalis were found to have the potential for mesenchymal differentiation. (C)2004 by American Society for Reproductive Medicine.