Effect of LED Phototherapy of Three Distinct Wavelengths on Fibroblasts on Wound Healing: A Histological Study in a Rodent Model
Effect of LED Phototherapy of Three Distinct Wavelengths on Fibroblasts on Wound Healing: A Histological Study in a Rodent Model
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DOI：
10.1089/pho.2009.2605
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发表时间：
2010-08-01
影响因子：
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通讯作者：
Pinheiro, Antonio L. B.
中科院分区：
文献类型：
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作者：
Cavalcanti de Sousa, Ana Paula;Santos, Jean N.;Pinheiro, Antonio L. B.
目的：观察三种波长发光二极管(LED)治疗前后背部皮肤创面成纤维细胞增殖的组织学变化。背景：成纤维细胞分泌伤口愈合所必需的物质。LED光疗对成纤维细胞增殖作用的研究报道很少，主要是体内研究。动物和方法：经巴伊亚联邦大学口腔医学院动物实验委员会批准后，取成年雄性Wistar大鼠16只，体重200~250g，在全身麻醉下于背部切开伤口，随机分为4组：G0组，未处理对照组；G1组，红色发光二极管(700+/-20 nm，15 mW，10J/cm(2))；G2，绿色LED(530+/-20 nm，8 mW，10J/cm(2))；G3，蓝光LED(460+/-20 nm，22 mW，10J/cm(2))。术后即刻开始照射，隔日重复照射，连续7天。术后8天处死动物。标本取出，常规加工成蜡，切片，苏木素/伊红(HE)染色。通过测量成纤维细胞在染色切片上占据伤口愈合相应区域的百分比来对成纤维细胞进行评分。结果：定量结果显示，与对照组相比，红色LED(700+/-20 nm)和绿色LED(530+/-20 nm)的成纤维细胞数量显著增加(p<0.01和p=0.02)。结论：使用绿色和红色LED灯可有效促进啮齿动物成纤维细胞的增殖。
Aim: The aim of the present investigation was to evaluate histologically fibroblastic proliferation on dorsal cutaneous wounds in a rodent model treated or not with light-emitting diodes (LEDs) of three wavelengths. Background: Fibroblasts secrete substances essential for wound healing. There are few reports of LED phototherapy on fibroblast proliferation, mainly in vivo. Animals and Methods: Following approval by the Animal Experimentation Committee of the School of Dentistry of the Federal University of Bahia, we obtained 16 young adult male Wistar rats weighing between 200 and 250 g. Under general anesthesia, one excisional wound was created on the dorsum of each animal; they were then randomly distributed into four groups of four animals each: G0, untreated control; G1, red LED (700 +/- 20 nm, 15mW, 10 J/cm(2)); G2, green LED (530 +/- 20 nm, 8mW, 10 J/cm(2)); and G3, blue LED (460 +/- 20 nm, 22mW, 10 J/cm(2)). The irradiation started immediately after surgery and was repeated every other day for 7 days. Animals were killed 8 days after surgery. The specimens were removed, routinely processed to wax, cut, and stained with hematoxylin/eosin (HE). Fibroblasts were scored by measuring the percentage of these cells occupying the area corresponding to wound healing on stained sections. Results: The quantitative results showed that red LED (700 +/- 20 nm) and green LED (530 +/- 20 nm) showed a significant increase in fibroblast numbers (p < 0.01 and p = 0.02) when compared with the control group. Conclusion: The use of green and red LED light is effective in increasing fibroblastic proliferation on rodents.