Comparison of bacterial microbiota in skin biopsies from normal and psoriatic skin
Comparison of bacterial microbiota in skin biopsies from normal and psoriatic skin
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DOI：
10.1007/s00403-011-1189-x
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发表时间：
2012-01-01
影响因子：
3
通讯作者：
Fry, Lionel
中科院分区：
文献类型：
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作者：
Fahlen, Annika;Engstrand, Lars;Fry, Lionel
银屑病的发病机制与微生物有关。以前对银屑病和正常皮肤的研究使用的是表面的拭子，而不是皮肤活检。在这项研究中，从10名银屑病患者和12名对照受试者的不匹配的网站采取活检。在靶向16 S rRNA基因和可变区V3-V4的454平台上用大规模平行焦磷酸测序分析样品。结果表明，这些样品共分为19门，265个分类单元和652个操作单位（OTU），同源性为97%。截止丰度水平设定为1%。正常皮肤和银屑病皮肤中最常见的三个门是厚壁菌门（39%银屑病，43%正常皮肤）、变形菌门（38%银屑病，27%正常皮肤）和放线菌门（5%银屑病，16%正常皮肤，p = 0.034）。在躯干皮肤中，与对照组相比，银屑病中变形菌门的水平显著更高（52%对32%，p = 0.0113）。银屑病（32%）和正常皮肤（26%）中最常见的属是链球菌。葡萄球菌在银屑病中（5%）比对照组（16%）更少见，丙酸杆菌也是如此（银屑病0.0001669%，对照组0.0254%）。银屑病患者的葡萄球菌和丙酸杆菌均显著低于对照肢体皮肤（分别为p = 0.051、0.046）。这项研究表明，银屑病和正常皮肤之间的微生物群存在一些差异。这些是否是原发性的病因学意义，或继发于银屑病的皮肤改变仍有待确定。
Microorganisms have been implicated in the pathogenesis of psoriasis. Previous studies of psoriasis and normal skin have used swabs from the surface rather than skin biopsies. In this study, biopsies were taken from 10 patients with psoriasis and 12 control subjects from unmatched sites. Samples were analysed with massive parallel pyrosequencing on the 454 platform targeting the 16S rRNA gene and the variable regions V3-V4. The samples grouped into 19 phyla, 265 taxon and 652 operational units (OTUs) at 97% identity. A cut-off abundance level was set at 1%. The three most common phyla in both normal and psoriasis skin were Firmicutes (39% psoriasis, 43% normal skin), Proteobacteria (38% psoriasis, 27% normal skin) and Actinobacteria (5% psoriasis, 16% normal skin, p = 0.034). In trunk skin, Proteobacteria were present at significantly higher levels in psoriasis compared to controls (52 vs. 32%, p = 0.0113). The commonest genera were Streptococci in both psoriasis (32%) and normal skin (26%). Staphylococci were less common in psoriasis (5%) than in controls (16%), as were Propionibacteria (psoriasis 0.0001669%, controls 0.0254%). Both Staphylococci and Propionibacteria were significantly lower in psoriasis versus control limb skin (p = 0.051, 0.046, respectively). This study has shown some differences in microbiota between psoriasis and normal skin. Whether these are of primary aetiological significance, or secondary to the altered skin of psoriasis remains to be determined.