A rapid method for sensitive profiling of folates from plant leaf by ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometer
A rapid method for sensitive profiling of folates from plant leaf by ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometer
复制标题
超高效液相色谱与串联四极杆质谱仪联用快速灵敏分析植物叶片叶酸的方法
DOI：
10.1016/j.jchromb.2016.11.033
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Yang, Xiaoe
中科院分区：
文献类型：
--
作者：
Shohag, M. J. I.;Yang, Qianying;Yang, Xiaoe
以前公布的方法用于分析叶酸是耗时的，因为冗长的样品提取，净化和总运行时间。本研究详细介绍了一种快速、灵敏和耐用的方法的开发和验证，该方法将简单的提取步骤与超高效液相色谱-串联四极杆质谱法相结合。本文报道了应用串联四极杆质谱仪分析植物源叶酸的最大七种维生素。通过线性、灵敏度、精密度、回收率试验和标准物质分析评价了该方法的分析性能。检测限和定量限范围为0.003和0.021微克/100克FW和0.011和0.041微克/100克FW，分别为回收率和旋进范围为71.27至99。01%和1.7 - 7.8%RSD，取决于叶酸维生素。这种新开发和验证的方法是快速的（色谱运行时间为5分钟），易于执行（没有费力和耗时的清理），并可用于同时分析从植物来源的叶酸的七种维生素。(C)2016爱思唯尔B. V.保留所有权利。
Previous published methods for the analysis of folates are time consuming because of lengthy sample extraction, clean-up and total running time. This study details the development and validation of a rapid, sensitive and robust method that combines a simple extraction step with ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometry. Here, we reported application of a tandem quadrupole mass spectrometer to analyze maximum seven vitamers of folate from plant origin. The analytical performance was evaluated by linearity, sensitivity, precision, recovery test and analysis of certified reference materials. The limit of detection and limit of quantification ranged between 0.003 and 0.021 mu g/100 g FW and between 0.011 and 0.041 mu g/100 g FW, respectively; the recovery and precession ranged from 71.27 to 99. 01% and from 1.7 to 7.8% RSD, respectively, depending upon folate vitamers. This newly developed and validated method is rapid (a chromatographic run time of 5 min), easy to be performed (no laborious and time consuming clean-up) and can be used to simultaneously analyze seven vitamers of folate from plant sources. (C) 2016 Elsevier B.V. All rights reserved.