Roles of the 5' leader region of the ompA mRNA.
Roles of the 5' leader region of the ompA mRNA.
复制标题
ompA mRNA 5 前导区的作用。
DOI：
10.1016/0022-2836(84)90499-6
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发表时间：
1984
影响因子：
5.6
通讯作者：
Inouye,M
中科院分区：
文献类型：
--
作者：
Green,PJ;Inouye,M
OmpA蛋白是大肠杆菌的一种主要外膜蛋白，由一个含有134个核苷酸的5′前导区的信使RNA合成。使用一系列ompA-lacZ融合质粒研究了该前导区在有效ompA表达中的作用。这些质粒在编码与lacZ结构基因融合的ompA前导区的DNA量上不同。含有ompAleader序列中除6个核苷酸外的所有核苷酸的融合体产生最高的β-半乳糖苷酶活性，而含有最短前导序列的融合体仅合成4%的β-半乳糖苷酶。融合与领导人中间的长度产生之间的6%和24%的活动中发现的最有效的融合。通过DNA-RNA杂交对lacZ mRNA合成的定量显示了lacZ mRNA产生的差异，反映了所观察到的β-半乳糖苷酶活性的差异。在mRNA二级结构的作用方面，讨论了在维持高水平的mRNA的主要作用的ompAleader。
OmpA protein, a major outer membrane protein ofEscherichia coli, is synthesized from a messenger RNA containing a 134-nucleotide 5′ leader region. The role of this leader region in efficientompAexpression was investigated using a series ofompA-lacZfusion plasmids. These plasmids differ in the amount of DNA encoding theompAleader region which is fused to thelacZstructural gene. The fusion containing all but six nucleotides of theompAleader produced the highest β-galactosidase activity, while the fusion containing the shortest leader synthesized only 4% as much β-galactosidase. Fusions with leaders intermediate in length produced between 6% and 24% of the activity found in the most efficient fusion. Quantitaten oflacZmRNA synthesis by DNA-RNA hybridization revealed differences inlacZmRNA production reflecting the observed differences in β-galactosidase activity. The primary effect of theompAleader in maintaining high levels of mRNA is discussed in terms of the roles of mRNA secondary structure.