Relation of activation-induced deaminase (AID) expression with antibody response to A(H1N1)pdm09 vaccination in HIV-1 infected patients
Relation of activation-induced deaminase (AID) expression with antibody response to A(H1N1)pdm09 vaccination in HIV-1 infected patients
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DOI：
10.1016/j.vaccine.2013.03.002
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发表时间：
2013-04-26
期刊：
影响因子：
5.5
通讯作者：
Chiodi, Francesca
中科院分区：
文献类型：
--
作者：
Cagigi, Alberto;Pensieroso, Simone;Chiodi, Francesca
先前已经指出了CD 4 + T细胞、病毒载量和年龄在HIV-1感染个体对流感感染和疫苗接种的免疫应答中的相关性。我们的研究旨在评估，在2009年A（H1N1）pdm 09流感疫苗接种的背景下，是否定量血液B细胞中的活化诱导脱氨酶（AID）表达可以提供额外的指标，用于预测具有相似CD 4 + T细胞计数和年龄的HIV-1感染患者对疫苗接种的抗体应答。47名健康对照，37名ART治疗和17名未经治疗的HIV-1感染患者参加了这项研究。在A（H1N1）pdm 09疫苗接种之前和疫苗接种后1、3和6个月收集血液。采用血凝抑制试验（HI）检测抗H1N1 pdm 09疫苗的抗体滴度，采用真实的时间定量PCR检测AID mRNA表达水平。在体外B细胞活化后，在所有个体中，AID增加与接种后1个月对A（H1N1）pdm 09疫苗的抗体应答相关。此外，在接种疫苗6个月后仍携带保护水平A（H1N1）pdm 09抗体的个体中，AID的最大表达水平显著更高。未发现CD 4 + T细胞计数或接种疫苗时的年龄或HIV-1病毒载量与A（H1N1）pdm 09抗体水平之间存在相关性。在免疫接种前评估AID表达可能是一个额外的有用的工具，用于定义免疫功能低下的个体在免疫失败的风险中的免疫接种策略。(c)2013爱思唯尔有限公司保留所有权利。
The relevance of CD4+T-cells, viral load and age in the immunological response to influenza infection and vaccination in HIV-1 infected individuals has previously been pointed out. Our study aimed at assessing, in the setting of 2009 A(H1N1)pdm09 influenza vaccination, whether quantification of activation-induced deaminase (AID) expression in blood B-cells may provide additional indications for predicting antibody response to vaccination in HIV-1 infected patients with similar CD4+T-cell counts and age. Forty-seven healthy controls, 37 ART-treated and 17 treatment-naive HIV-1 infected patients were enrolled in the study. Blood was collected prior to A(H1N1)pdm09 vaccination and at 1, 3 and 6 months after vaccination. Antibody titers to A(H1N1)pdm09 vaccine were measured by hemagglutination inhibition (HI) assay while the mRNA expression levels of AID were measured by quantitative real time PCR. Upon B-cell activation in vitro, AID increase correlated to antibody response to the A(H1N1)pdm09 vaccine at 1 month after vaccination in all individuals. In addition, the maximum expression levels of AID were significantly higher in those individuals who still carried protective levels of A(H1N1)pdm09 antibodies after 6 months from vaccination. No correlation was found between CD4+T-cell counts or age at vaccination or HIV-1 viral load and levels of A(H1N1)pdm09 antibodies. Assessing AID expression before vaccination may be an additional useful tool for defining a vaccination strategy in immune-compromised individuals at risk of immunization failure. (c) 2013 Elsevier Ltd. All rights reserved.