EFFECT OF TEMPERATURE ON VIABILITY OF BACILLUS-STEAROTHERMOPHILUS
EFFECT OF TEMPERATURE ON VIABILITY OF BACILLUS-STEAROTHERMOPHILUS
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DOI：
10.1007/bf00427870
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发表时间：
1976-01-01
影响因子：
2.8
通讯作者：
NOSOH, Y
中科院分区：
文献类型：
--
作者：
HASHIZUME, S;SEKIGUCHI, T;NOSOH, Y
其中一种专性嗜热细菌，嗜热硬脂芽孢杆菌，不能在低于35℃的温度下生长。在这种温度下，培养的细菌中约有80%的人死亡，并且在生物体的氧气消耗活动或蛋白质或核酸合成方面也有相同程度的损失。随着生物体死亡的进行，还原性的烟酰胺-腺嘌呤二核苷酸被生物体氧化，用磷酸盐缓冲液洗涤生物体时，果糖-1,6-二磷酸醛缩酶等酶从生物体中泄漏出来，越来越多的核糖核蛋白被释放到培养基中。膜状态的变化被认为是生物在高温下死亡的可能原因之一。
One of the obligate thermophilic bacteria,Bacillus stearothermophilus, was unable to grow at temperatures below 35° C. About 80% of the population in the bacterial culture died at the temperatures, and the same extent of loss in either of the activities of oxygen consumption or synthesis of protein or nucleic acid of the organisms was observed. With the progress of death of the organisms, reduced nicotinamide-adenine dinucleotide came to be oxidized by the organisms, enzymes such as fructose-1,6-diphosphate aldolase, when the organisms were washed with phosphate buffer, were leaked out of the organisms, and an increasing amount of ribonucleoprotein was released into the culture medium. The change of the membrane state was then suggested to be one of the possible causes for the death of the organisms at the temperatures.