Carrier of reduced sulfur is a possible role for thiotaurine in symbiotic species from hydrothermal vents with thiotrophic symbionts
Carrier of reduced sulfur is a possible role for thiotaurine in symbiotic species from hydrothermal vents with thiotrophic symbionts
复制标题
还原硫的载体是硫牛磺酸在来自热液喷口与硫营养共生体的共生物种中的可能作用
作者：
A. Pruski;R. De Wit;A. Fiala
实验支持可能的作用的游离含硫氨基酸硫代牛磺酸，作为运输和存储化合物的硫化物在无脊椎动物与硫营养共生体的描述。自由生活的化能营养型硫氧化细菌，Thiobacillus hydrothermalis（菌株DSMZ 7121），被用作共生体的模型，因为实际的共生体还没有得到在culture.Thiotaurine含有两个硫原子，即内砜和外硫烷硫，后者提出了一个潜在的来源，为共生体的还原当量。尽管如此，当将该化合物加入T.水热法在硫化物上预生长。相比之下，当硫代牛磺酸与马努斯盆地的vestimentiferan管虫的滋养体的提取物一起加入到培养物中时，我们观察到硫代牛磺酸被氧化为亚牛磺酸，伴随着酸化和细菌生物量的形成。因此，滋养体含有未知的催化因子。我们认为，硫代牛磺酸需要还原之前，氧化T。水热合成法和主体可以催化硫代牛磺酸通过谷胱甘肽氧化还原对的转化。通过这种方式，宿主可以精确地控制向共生体的能量输送（如还原硫），因此可以控制它们的共生体生物量。
Experiments supporting the possible role of the free sulfur-containing amino acid thiotaurine, as a transport and storage compound for sulfide in invertebrates with thiotrophic symbionts are described. The free-living chemotrophic sulfur-oxidising bacterium, Thiobacillus hydrothermalis (strain DSMZ 7121), was used as a model for the symbionts as the actual symbionts have not been obtained in culture.Thiotaurine contains two sulfur atoms, namely the inner sulfone and the outer sulfane sulfur; the latter presents a potential source of reducing equivalents for the symbiont. Nevertheless, we observed no oxidation of thiotaurine when this compound was added to a culture of T. hydrothermalis pre-grown on sulfide. In contrast, when thiotaurine was added to the culture together with an extract of the trophosome of a vestimentiferan tubeworm from the Manus basin, we observed that thiotaurine was oxidised to hypotaurine with concomitant acidification and formation of bacterial biomass. Thus, the trophosome contains an unknown catalytic factor. We suggest that thiotaurine requires reduction prior to oxidation by T. hydrothermalisand that the host may catalyse the conversion of thiotaurine through the glutathione redox couple. This way, the host can accurately control energy delivery (as reduced sulfur) to the symbionts and can therefore control their symbiont biomass.
酵母硫代硫酸还原酶的催化机制。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chauncey,TR;Westley,J
通讯作者：
Westley,J