Seasonal analysis of protistan community structure and diversity at the USC Microbial Observatory (San Pedro Channel, North Pacific Ocean)
Seasonal analysis of protistan community structure and diversity at the USC Microbial Observatory (San Pedro Channel, North Pacific Ocean)
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DOI：
10.4319/lo.2010.55.6.2381
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发表时间：
2010-11-01
影响因子：
4.5
通讯作者：
Caron, David A.
中科院分区：
文献类型：
--
作者：
Countway, Peter D.;Vigil, Patrick D.;Caron, David A.
在美国南加州海岸外的太平洋时间序列站，对海洋原生生物群落的结构和遗传多样性进行了研究。2001年1月、4月、7月和10月在4个深度（5 m、叶绿素最大值[CM]、150 m和500 m）研究了基于脱氧核糖核酸序列的微生物真核生物多样性。2956个部分18S核糖体核糖核酸基因序列产生了大多数真核生物主要谱系的代表。在分类类群中值得注意的是最近描述的层叠虫、肺泡虫和裸藻的谱系。在深度处观察到大量的多胱氨酸和棘细胞序列。两两序列分析建立了操作分类单位（otu），并通过参数和非参数技术估计了未采样原生生物的多样性。2246个原生物序列被划分为377个不同的otu，其余序列归属于后生动物。当将所有深度和季节组合成单一数据集时，原生生物丰富度估计范围为600至1500 otu。从单变量和多变量分析比较，观察到的原生生物多样性的季节和深度相关趋势是明显的。聚类分析结合非度量多维标度和相似性检验分析发现，各季节最浅深度（5 m和CM）的原生生物组合不同，与最深深度（150 m和500 m）的原生生物组合差异显著（p < 0.03），其中原生生物组合季节变化不明显。
The structure and genetic diversity of marine protistan assemblages were investigated in the upper 500 m of the water column at a Pacific Ocean time-series station off the coast of Southern California. Deoxyribonucleic acid sequence-based microbial eukaryote diversity was examined in January, April, July, and October of 2001 at four depths (5 m, chlorophyll maximum [CM], 150 m, and 500 m). A total of 2956 partial 18S ribosomal ribonucleic acid gene sequences yielded representatives from most of the major eukaryotic lineages. Notable among the taxonomic groups were recently described lineages of stramenopiles, alveolates, and euglenozoa. A large number of polycystine and acantharean sequences were observed at depth. Pairwise sequence analysis was performed to establish operational taxonomic units (OTUs) that were then used to estimate the unsampled protistan diversity by parametric and nonparametric techniques. A total of 2246 protistan sequences grouped into 377 distinct OTUs, with remaining sequences attributed to metazoa. Protistan richness estimates ranged from, 600 to 1500 OTUs when all depths and seasons were combined into a single data set. Seasonal and depth-related trends in the observed protistan diversity were apparent from comparisons of univariate and multivariate analyses. Cluster analysis combined with nonmetric multidimensional scaling and analysis of similarity testing identified distinct protistan assemblages at the shallowest depths (5 m and CM) for each season, which were significantly different (p < 0.03) from assemblages at the two deepest depths (150 and 500 m) where seasonal changes in the protistan assemblage were not apparent.