The Cytosolic/Nuclear HSC70 and HSP90 Molecular Chaperones Are Important for Stomatal Closure and Modulate Abscisic Acid-Dependent Physiological Responses in Arabidopsis
The Cytosolic/Nuclear HSC70 and HSP90 Molecular Chaperones Are Important for Stomatal Closure and Modulate Abscisic Acid-Dependent Physiological Responses in Arabidopsis
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DOI：
10.1104/pp.111.174425
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发表时间：
2011-07-01
期刊：
影响因子：
7.4
通讯作者：
Noel, Laurent D.
中科院分区：
文献类型：
--
作者：
Clement, Mathilde;Leonhardt, Nathalie;Noel, Laurent D.
热休克蛋白家族HSP 90和HSC 70的胞质/核分子伴侣是真核生物中保守的必需蛋白。这些蛋白质在高等植物的先天免疫和非生物胁迫耐受中起着重要作用。在这里，我们表明，这两个分子伴侣在拟南芥（拟南芥）中招募气孔关闭诱导的几个环境信号。过表达HSC 70 -1或HSP 90.2活性降低的植物在黑暗、CO2、鞭毛蛋白22肽和脱落酸（阿坝）诱导的气孔关闭中受到损害。HSC 70 -1和HSP 90蛋白需要建立几个ABA响应基因的基础表达水平，这表明这些分子伴侣也可能参与阿坝信号传导事件。植物过表达HSC 70 -1或减少HSP 90.2活性是超敏感的阿坝在种子萌发试验中，这表明几个分子伴侣复合物与不同的基板可能会调整组织特异性响应阿坝和其他生物和非生物刺激的研究。这项研究表明，HSC 70/HSP 90机制是重要的气孔关闭，并在植物中提供必要的功能，以整合来自生物和非生物环境的信号。
Cytosolic/nuclear molecular chaperones of the heat shock protein families HSP90 and HSC70 are conserved and essential proteins in eukaryotes. These proteins have essentially been implicated in the innate immunity and abiotic stress tolerance in higher plants. Here, we demonstrate that both chaperones are recruited in Arabidopsis (Arabidopsis thaliana) for stomatal closure induced by several environmental signals. Plants overexpressing HSC70-1 or with reduced HSP90.2 activity are compromised in the dark-, CO2-, flagellin 22 peptide-, and abscisic acid (ABA)-induced stomatal closure. HSC70-1 and HSP90 proteins are needed to establish basal expression levels of several ABA-responsive genes, suggesting that these chaperones might also be involved in ABA signaling events. Plants overexpressing HSC70-1 or with reduced HSP90.2 activity are hypersensitive to ABA in seed germination assays, suggesting that several chaperone complexes with distinct substrates might tune tissue-specific responses to ABA and the other biotic and abiotic stimuli studied. This study demonstrates that the HSC70/HSP90 machinery is important for stomatal closure and serves essential functions in plants to integrate signals from their biotic and abiotic environments.