Genetic variation and widespread dispersal of Nosema ceranae in Apis mellifera apiaries from Argentina
Genetic variation and widespread dispersal of Nosema ceranae in Apis mellifera apiaries from Argentina
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DOI：
10.1007/s00436-011-2566-2
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发表时间：
2012-02-01
影响因子：
2
通讯作者：
Javier Eguaras, Martin
中科院分区：
文献类型：
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作者：
Karina Medici, Sandra;Gabriel Sarlo, Edgardo;Javier Eguaras, Martin
使用分子技术，我们记录了来自阿根廷的蜜蜂(Apis Melvena)中存在脑微孢子虫。样本采集自阿根廷布宜诺斯艾利斯省38个地区的意大利蜜蜂群体。分子鉴定是通过一种基于多重PCR的方法实现的，该方法允许并行诊断中华绒毛虫和中华绒毛虫原料药。在所分析的所有样品中都检出了中华绒毛虫。此外，在Balcarce和Maipa(0)区发现了与N.api混合感染。我们鉴定了中华蜜蜂的三个rRNA序列变异体，它们可能代表了蜜蜂输入的不同来源。这些结果表明，中华绒毛虫病在阿根廷广泛分布，不同分离株之间的遗传差异可能与我们工作组先前发现的症状学上的差异有关。我们的结果突显了重新评估当前有效的健康方案的必要性，以便它们认识到中华绒毛虫是这个国家诺森氏菌病的主要致病因素。
Using molecular techniques, we documented the presence of Nosema ceranae in honeybees (Apis mellifera) from Argentina. Samples were collected from A. mellifera colonies in 38 districts of Buenos Aires province, Argentina. Molecular characterization was achieved with a multiplex PCR-based method, which allows parallel diagnosis of N. ceranae and N. osema apis. N. ceranae was identified in all the samples analyzed. Moreover, coinfections with N. apis were detected in Balcarce and MaipA(0) districts. We identified three rRNA sequence variants of N. ceranae, which may represent diverse sources of bee importation. The results suggest that N. ceranae is widely distributed in Argentina and that the genetic variation observed between the different isolates could be related with the difference in the symptomatology found previously by our work group. Our results highlight the need to re-assess the health protocols currently in force so that they recognize N. ceranae as the main causal agent of Nosemosis in this country.