Ly49B is expressed on multiple subpopulations of myeloid cells
Ly49B is expressed on multiple subpopulations of myeloid cells
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DOI：
10.4049/jimmunol.177.9.5840
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发表时间：
2006-11-01
影响因子：
4.4
通讯作者：
Brooks, Colin G.
中科院分区：
文献类型：
--
作者：
Gays, Frances;Aust, Jonathan G.;Brooks, Colin G.
使用一种新的mAb特异性小鼠Ly 49 B，我们在这里报告说，Ly 49 B，最后剩下的成员C57 Ly 49家族的特点，是在低水平上表达类似于1.5%的脾细胞，没有这是NK细胞或T细胞，而是属于几个不同的亚群的骨髓细胞定义的表达CD 11b和不同水平的Gr 1。更大比例的骨髓和腹膜细胞表达Ly 49 B，均为CD 11b（+），并包含由光散射、F4/80和GrI表达定义的多个亚群。Ly 49 Q也在髓样细胞上表达，共染色显示Ly 49 B和Ly 49 Q在非重叠亚群上表达最强，Ly 49 Q（高）细胞主要是B220（+）CD 4（+）和/或CD 8（+），Ly 49 B（+）细胞缺乏这些标记。在体外从骨髓祖细胞发育的髓样细胞群体经常共表达Ly 49 B和Ly 49 Q，并且Ly 49 B的表达可以被LPS、α-IFN和γ-IFN上调，通常独立于Ly 49 Q。PCR分析显示，培养的NK细胞和T细胞含有Ly 49 B转录本，并且在IL-12加IL-18中培养的NK细胞上和未成熟的NK细胞系上可以检测到Ly 49 B表达。免疫组织化学研究表明，Ly 49 B在组织中的表达重叠，但不同于所有其他髓样分子的检查，是特别突出的固有层和圆顶的派尔集合淋巴结，暗示一个重要的作用，Ly 49 B在肠道免疫生物学。在转染的细胞中，发现Ly 49 B与SHP-1，SHP-2和SHIP以受细胞内磷酸化事件强烈调节的方式相关联。
Using a novel mAb specific for mouse Ly49B, we report here that Ly49B, the last remaining member of the C57 Ly49 family to be characterized, is expressed at low levels on similar to 1.5% of spleen cells, none which are NK cells or T cells but which instead belong to several distinct subpopulations of myeloid cells defined by expression of CD11b and different levels of Gr1. Much larger proportions of bone marrow and peritoneal cells expressed Ly49B, all being CD11b(+) and comprising multiple subpopulations defined by light scatter, F4/80, and GrI expression. Costaining for Ly49Q, also expressed on myeloid cells, revealed that Ly49B and Ly49Q were most strongly expressed on nonoverlapping subpopulations, Ly49Q(high) cells being mostly B220(+)CD4(+) and/or CD8(+), Ly49B(+) cells lacking these markers. Myeloid populations that developed from bone marrow progenitors in vitro frequently coexpressed both Ly49B and Ly49Q, and Ly49B expression could be up-regulated by LPS, alpha-IFN, and gamma-IFN, often independently of Ly49Q. PCR analysis revealed that cultured NK cells and T cells contained Ly49B transcripts, and Ly49B expression could be detected on NK cells cultured in IL-12 plus IL-18, and on an immature NK cell line. Immunohistochemical studies showed that Ly49B expression in tissues overlapped with but was distinct from that of all other myeloid molecules examined, being particularly prominent in the lamina propria and dome of Peyer's patches, implicating an important role of Ly49B in gut immunobiology. In transfected cells, Ly49B was found to associate with SHP-1, SHP-2, and SHIP in a manner strongly regulated by intracellular phosphorylation events.