Hippocampal neuronal maturation triggers post-synaptic clustering of brain temperature-sensor TRPV4
Hippocampal neuronal maturation triggers post-synaptic clustering of brain temperature-sensor TRPV4
复制标题
海马神经元成熟触发大脑温度传感器 TRPV4 的突触后聚集
DOI：
10.1016/j.bbrc.2015.01.087
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ishizaki Y.
中科院分区：
文献类型：
--
作者：
Shibasaki K;Tominaga M;Ishizaki Y.
神经元功能的区室化是通过离散亚细胞膜域中离子通道的特定局部聚集来实现的。瞬时受体电位 (TRP) 通道表现出高度可变的细胞和亚细胞表达模式。我们之前揭示了热传感器 TRPV4（在 34°C 以上激活）由海马神经元的生理脑温度控制，从而控制它们的兴奋性。在这里，我们检查了 TRPV4 在发育中的海马神经元中的突触聚类。我们发现 TRPV4 在未成熟海马神经元的体细胞中积累，尽管 PSD-95 标记的突触后结构很明显，但并未定位于突触后位置。在神经元成熟过程中，TRPV4 靶向树突并聚集在突触后位置。综上所述，我们揭示了 TRPV4 定位于突触后位点，并且突触后靶向以神经元成熟依赖性方式受到严格调节。
Compartmentalization of neuronal function is achieved via specifically localized clustering of ion channels in discrete subcellular membrane domains. Transient receptor potential (TRP) channels exhibit highly variable cellular and subcellular patterns of expression. We previously revealed that the thermo-sensor TRPV4 (activated above 34 °C) is gated by physiological brain temperatures in hippocampal neurons and thereby controls their excitability. Here, we examined synaptic clustering of TRPV4 in developing hippocampal neurons. We found that TRPV4 accumulated in the soma of immature hippocampal neurons, and did not localize to post-synaptic locations although PSD-95-labeled post-synaptic structures were evident. During the maturation of neurons, TRPV4 was targeted to dendrites and also clustered at post-synaptic locations. Taken together, we reveal that TRPV4 localizes to post-synaptic sites and the post-synaptic targeting is strictly regulated in a neuronal maturation-dependent manner.