LOCALIZATION OF SPECIFICITY DETERMINING SITES IN CADHERIN CELL-ADHESION MOLECULES
LOCALIZATION OF SPECIFICITY DETERMINING SITES IN CADHERIN CELL-ADHESION MOLECULES
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DOI：
10.1016/0092-8674(90)90222-z
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发表时间：
1990-04-06
期刊：
影响因子：
64.5
通讯作者：
TAKEICHI, M
中科院分区：
文献类型：
--
作者：
NOSE, A;TSUJI, K;TAKEICHI, M
钙粘蛋白是一组嗜同性细胞间粘附分子;该家族的每个成员都表现出结合特异性。在这里，我们试图通过分析表达嵌合和点突变的E-和P-钙粘蛋白的细胞的粘附选择性来绘制这些分子的特异性的位点。结果表明，氨基末端113个氨基酸的区域是必不可少的，以确定的特异性，在这个区域内，我们可以确定特别重要的网站，其中氨基酸取代改变了钙粘蛋白的结合特异性。我们还发现能够阻断钙粘蛋白作用的抗体的表位位于该氨基末端区域。
Cadherins are a group of homophilic intercellular adhesion molecules; each member of this family exhibits binding specificity. Here, we attempted to map the sites for the specificities of these molecules by analyzing adhesive selectivities of the cells that express chimeric and point-mutated E- and P-cadherin. The results showed that the amino-terminal 113 amino acid region is essential to determine the specificities, and within this region we could identify especially important sites in which amino acid substitutions altered the binding specificity of cadherins. We also found that the epitopes for antibodies capable of blocking cadherin action are located in this amino-terminal region.