REGULATORY GENE EVOLUTION - ADAPTIVE DIFFERENCES IN EXPRESSION OF ALCOHOL-DEHYDROGENASE IN DROSOPHILA-MELANOGASTER AND DROSOPHILA-SIMULANS
REGULATORY GENE EVOLUTION - ADAPTIVE DIFFERENCES IN EXPRESSION OF ALCOHOL-DEHYDROGENASE IN DROSOPHILA-MELANOGASTER AND DROSOPHILA-SIMULANS
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DOI：
10.1038/hdy.1984.23
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发表时间：
1984-01-01
期刊：
影响因子：
3.8
通讯作者：
BRENNAN, MD
中科院分区：
文献类型：
--
作者：
DICKINSON, WJ;ROWAN, RG;BRENNAN, MD
解析：选d。拟南芥杂交后，两亲本的乙醇脱氢酶（ADH）电形态特征在相对表达水平上表现出组织和阶段特异性差异。这意味着不同的顺式调控元件与各自的Adh等位基因相关。这些顺式作用元件部分地，但不是完全地，解释了在两个物种中ADH表达的显著不同的总体模式。调控模式似乎是适应性显著的，因为它们与物种特异性的乙醇耐受性模式相关。活性差异是由不同水平的酶蛋白引起的，但其潜在机制尚未得到充分分析，可能很复杂。ADH发育过程各方面的独立进化可能与不同发育背景下ADH基因座转录使用不同启动子有关。该系统说明了调控基因在进化中的潜在重要性，并为研究进化调控差异的分子基础提供了一个模型。
In D. melanogaster .times. D. simulans hybrids, the alcohol dehydrogenase (ADH) electromorphs characteristic of the 2 parents display tissue- and stage-specific differences in relative level of expression. This implies distinct cis-acting regulatory elements associated with the respective Adh alleles. These cis-acting elements account in part, but not completely, for markedly different overall patterns of ADH expression in the 2 species. The regulatory patterns seem to be adaptively significant since they correlate with species-specific patterns of ethanol tolerance. The activity differences are accounted for by different levels of enzyme protein, but the underlying mechanisms were not fully analyzed and may be complex. Independent evolution of various aspects of the ADH developmental program may relate to use of different promoters for transcription of the Adh locus in different developmental contexts. This system illustrates the potential importance of regulatory genes in evolution and provides a model for investigating the molecular basis of evolved regulatory differences.