In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating
In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating
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DOI：
10.1016/j.biomaterials.2006.07.003
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发表时间：
2006-11-01
期刊：
影响因子：
14
通讯作者：
Ong, J. L.
中科院分区：
文献类型：
--
作者：
Chen, W.;Liu, Y.;Ong, J. L.
种植体植入后细菌感染是一个显著上升的并发症。为了降低植入物相关感染的发生率，已经提出了几种生物材料表面处理。研究了共溅射含银（Ag）的羟基磷灰石（HA）涂层的体外抗菌活性和细胞毒性。通过同时向Ag靶供应10 W和向HA靶供应300 W来实现沉积。在400摄氏度下热处理4小时。X射线衍射，接触角测量，和表面粗糙度被用来表征涂层表面。RP 12菌株的表皮葡萄球菌（ATCC 35984）和科万I菌株的金黄色葡萄球菌被用来评价抗菌活性的Ag-HA涂层，而人胚胎腭间充质细胞，成骨细胞前体细胞系，被用来评价在体外细胞毒性的涂层。在这项研究中进行的X-射线衍射分析表明，对应于Ag和HA的共溅射Ag-HA表面上的峰。观察到HA和Ag-HA表面的接触角显著低于Ti表面，而所有组的表面粗糙度均无显著差异。体外细菌粘附研究表明，S。表皮葡萄球菌和表皮葡萄球菌。与钛（Ti）和HA表面相比，Ag-HA表面上的金黄色葡萄球菌。此外，在HA和Ag-HA表面之间没有观察到体外细胞毒性的显著差异。总之，得出的结论是，可以通过共溅射骨传导性HA与抗菌Ag来实现多功能表面的创建。(c)2006爱思唯尔有限公司保留所有权利。
Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co-sputtered silver (Ag)-containing hydroxyapatite (HA) coating was evaluated. Deposition was achieved by a concurrent supply of 10 W to the Ag target and 300 W to the HA target. Heat treatment at 400 degrees C for 4 It was performed after 3 h deposition. X-ray diffraction, contact angles measurements, and surface roughness were used to characterize the coating surfaces. The RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus were used to evaluate the antibacterial activity of the Ag-HA coatings, whereas human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the in vitro cytotoxicity of the coatings. X-ray diffraction analysis performed in this study indicated peaks corresponding to Ag and HA on the co-sputtered Ag-HA surfaces. The contact angles for HA and Ag-HA surfaces were observed to be significantly lower when compared to Ti surfaces, whereas no significant difference in surface roughness was observed for all groups. In vitro bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on Ag-HA surface when compared to titanium (Ti) and HA surfaces. In addition, no significant difference in the in vitro cytotoxicty was observed between HA and Ag-HA surfaces. Overall, it was concluded that the creation of a multifunctional surface can be achieved by co-sputtering the osteoconductive HA with antibacterial Ag. (c) 2006 Elsevier Ltd. All rights reserved.