Characterization of Holstein and Normande whole milk miRNomes highlights breed specificities
Characterization of Holstein and Normande whole milk miRNomes highlights breed specificities
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DOI：
10.1038/s41598-019-56690-7
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发表时间：
2019-12-30
影响因子：
4.6
通讯作者：
Le Provost, F.
中科院分区：
文献类型：
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作者：
Le Guillou, S.;Leduc, A.;Le Provost, F.
牛奶作为一种健康食品的概念为牛奶成分的研究开辟了道路，从营养物质到microRNA，牛奶中大量存在的具有广泛调节特性的分子。根据泌乳阶段，已在几个物种（如人和牛）中对这些组分进行了表征。在这里，我们根据遗传背景研究了牛奶microRNA组成的变化。使用高通量测序，我们对具有不同产奶特征的奶牛品种荷斯坦牛和诺曼底牛的牛奶miRNomes进行了表征和比较，以突出对调节哺乳过程至关重要的microRNA。在荷斯坦奶牛和诺曼底奶牛的乳汁中，分别鉴定出2,038种和2,030种microRNA，共有1,771种microRNA，其中1,049种被注释，722种被预测。两个品种的牛奶miRNome的比较允许突出显示182个microRNA，其在丰度上显示出显著差异。它们参与调节脂质代谢和乳腺形态发生和发育，从而影响泌乳。我们的研究结果提供了新的见解参与牛奶生产的分子机制的调节。
The concept of milk as a healthy food has opened the way for studies on milk components, from nutrients to microRNAs, molecules with broad regulatory properties present in large quantities in milk. Characterization of these components has been performed in several species, such as humans and bovine, depending on the stages of lactation. Here, we have studied the variation in milk microRNA composition according to genetic background. Using high throughput sequencing, we have characterized and compared the milk miRNomes of Holstein and Normande cattle, dairy breeds with distinct milk production features, in order to highlight microRNAs that are essential for regulation of the lactation process. In Holstein and Normande milk, 2,038 and 2,030 microRNAs were identified, respectively, with 1,771 common microRNAs, of which 1,049 were annotated and 722 were predicted. The comparison of the milk miRNomes of two breeds allowed to highlight 182 microRNAs displaying significant differences in the abundance. They are involved in the regulation of lipid metabolism and mammary morphogenesis and development, which affects lactation. Our results provide new insights into the regulation of molecular mechanisms involved in milk production.