IFN-γ mediates graft-versus-breast cancer effects via enhancing cytotoxic T lymphocyte activity.
IFN-γ mediates graft-versus-breast cancer effects via enhancing cytotoxic T lymphocyte activity.
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IFN-γ 通过增强细胞毒性 T 淋巴细胞活性来介导移植物抗乳腺癌效应。
DOI：
10.3892/etm.2014.1760
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发表时间：
2014-08
影响因子：
2.7
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Zhao Q;Tong L;He N;Feng G;Leng L;Sun W;Xu Y;Wang Y;Xiang R;Li Z
Previous studies have demonstrated the beneficial effect of graft-versus-tumor (GVT) following hematopoietic stem cell transplantation (HSCT) on the incidence of leukemia relapse and the overall survival rate of patients with leukemia; however, detailed mechanisms underlying the effects GVT exhibits on solid tumors following allogeneic HSCT are yet to be elucidated. The aim of the present study was to investigate the immune mechanism underlying the effect of interferon (IFN)-γ on GVT following allogeneic HSCT in breast cancer therapy. An in situ breast cancer mouse model was established by injecting 5×104 4T1 cells into the mammary fat pads of BALB/c mice. The 4T1 cells were transfected with the firefly luciferase reporter gene in order to monitor the tumor progression in real time. An allogeneic HSCT model was then established by transplanting bone marrow mononuclear cells from C57BL/6 mice to the BALB/c mice. To investigate the influence of T lymphocyte proliferation following allogeneic bone marrow transplantation, the levels of CD3+CD8+ cytotoxic T lymphocytes (CTLs) and CD4+CD25+ regulatory T cells were determined. In addition, IFN-γ and granzyme B expression levels in splenic lymphocytes were analyzed using flow cytometry. Allogeneic HSCT was found to significantly promote the proliferation and cytotoxicity of CTLs and suppress the growth of breast cancer. Furthermore, the secretory levels of IFN-γ and granzyme B by T cells were elevated following allogeneic HSCT. These results indicated that alloreactive T cells increased the secretion of IFN-γ, which promoted the alloresponse of donor CTLs. In addition, the CTLs produced granzyme B, which exerted a tumor suppressive effect.
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影响因子：
20.3
作者：
Taylor, PA;Lees, CJ;Blazar, BR
通讯作者：
Blazar, BR
影响因子：
4.3
作者：
Asavaroengchai, Wannee;Wang, Hui;Yang, Yong-Guang
通讯作者：
Yang, Yong-Guang
影响因子：
20.3
作者：
Yang, YG;Qi, J;Sykes, M
通讯作者：
Sykes, M
DOI：
10.1038/nri3212
发表时间：
2012-05-11
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.5
作者：
Gillmore, R;Xue, SA;Stauss, HJ
通讯作者：
Stauss, HJ