Comparison of Circulating Plasma DNA Levels between Lung Cancer Patients and Healthy Controls
Comparison of Circulating Plasma DNA Levels between Lung Cancer Patients and Healthy Controls
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DOI：
10.2353/jmoldx.2009.080098
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发表时间：
2009-05-01
影响因子：
4.1
通讯作者：
Lee, Jin Soo
中科院分区：
文献类型：
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作者：
Yoon, Kyong-Ah;Park, Sohee;Lee, Jin Soo
虽然肿瘤来源的细胞游离DNA的生物学功能以前还没有被阐明，但突变，超甲基化和微卫星不稳定性等分子特征已证实其肿瘤起源。因此，为了研究血浆DNA水平作为肺癌生物标志物的用途，我们使用定量PCR分析比较了102名肺癌患者和105名健康个体的血浆DNA浓度。健康组和癌症组的中位血浆DNA浓度分别为10.4和22.6 ng/ml（P < 0.0001），在I期或II期疾病患者中也检测到血浆DNA水平升高。无论是吸烟状况还是每年的吸烟量，都不会对循环中的游离DNA水平产生影响。循环游离DNA浓度的增加显示了区分肺癌的潜在能力（受试者工作特征曲线下面积= 0.86，95% CI = 0.81至0.91）。当受试者根据其血浆DNA浓度分为三组时，与最低三分位数的受试者相比，上三分位数（即具有最高浓度的受试者）的肺癌风险显著增加（校正比值比= 50.6，P < 0.001）。这些结果表明，循环血浆DNA水平升高可能作为一个潜在的诊断指标，是肺癌的一个重要的危险因素。（J Mol Diagn 2009，11：182-185; DOI：10.2353/jmoldx.2009.080098）
Although the biological functions of tumor-originated cell-free DNA have not been previously clarified, such molecular characteristics as mutations, hypermethylation, and microsatellite instability have confirmed its tumor origin. Therefore, to investigate the use of plasma DNA level as a biomarker of lung cancer, we compared plasma DNA concentrations in 102 patients with lung cancer and 105 healthy individuals using quantitative PCR analyses. The median plasma DNA concentrations for the healthy and cancer groups were 10.4 and 22.6 ng/ml, respectively (P < 0.0001), and elevated plasma DNA levels were also detected in patients with either stage I or II disease. Neither smoking status nor the number of packs per year had an effect on the level of circulating cell-free DNA. Increased concentrations of circulating cell-free DNA showed the potential power to discriminate lung cancer (area under the receiver operating characteristic curve = 0.86, 95% CI = 0.81 to 0.91). When subjects were classified into three groups based on their plasma DNA concentrations, subjects in the upper tertile (ie, those with the highest concentration) had a significantly increased risk of lung cancer as compared with those in the lowest tertile (adjusted odds ratio = 50.6, P < 0.001). These results suggest that elevated circulating plasma DNA levels may serve as a potential diagnostic indicator and be an important risk factor for lung cancer. (J Mol Diagn 2009, 11:182-185; DOI: 10.2353/jmoldx.2009.080098)