Defluviitoga tunisiensis gen. nov., sp nov., a thermophilic bacterium isolated from a mesothermic and anaerobic whey digester
Defluviitoga tunisiensis gen. nov., sp nov., a thermophilic bacterium isolated from a mesothermic and anaerobic whey digester
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DOI：
10.1099/ijs.0.033720-0
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Fardeau, Marie-Laure
中科院分区：
文献类型：
--
作者：
Ben Hania, Wajdi;Godbane, Ramzi;Fardeau, Marie-Laure
菌株SulfLac1(T)是从突尼斯的一个乳清消化器中分离到的一株外部结构类似鞘的嗜热、厌氧、轻度嗜盐的杆状细菌(TOGA)。该菌株的非运动细胞大小为3-30×1微米，呈单个、成对或长链状。新菌株将硫代硫酸盐和元素硫还原为硫化物，而不是硫酸盐或亚硫酸盐。它在37-65℃(最适55℃)、pH 6.5-7.9(最适pH 6.9)和0.2-3%(w/v)的氯化钠(最适0.5%)条件下生长。该菌株基因组DNA的G+C含量为33.6mol%。基于16S rRNA基因序列的系统发育分析表明，菌株SulfLac1(T)与Petrotoga mobilis的亲缘关系最近，序列相似性为91.4%。根据表型、系统发育和化学分类的证据，SulfLac1(T)菌株是一新种，隶属于热风菌目，命名为Defluviitoga tunisiens gen。Nov.，sp.11月是提议的。模式种的模式株为Sulflac1(T)(=Dsm 23805(T)=Jcm 17210(T))。
Strain SulfLac1(T), a thermophilic, anaerobic and slightly halophilic, rod-shaped bacterium with a sheath-like outer structure (toga), was isolated from a whey digester in Tunisia. The strain's non-motile cells measured 3-30x1 mu m and appeared singly, in pairs or as long chains. The novel strain reduced thiosulfate and elemental sulfur, but not sulfate or sulfite, into sulfide. It grew at 37-65 degrees C (optimum 55 degrees C), at pH 6.5-7.9 (optimum pH 6.9) and with 0.2-3% (w/v) NaCl (optimum 0.5 %). The G +C content of the strain's genomic DNA was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SulfLac1(T) was most closely related to Petrotoga mobilis (91.4% sequence similarity). Based on phenotypic, phylogenetic and chemotaxonomic evidence, strain SulfLac1(T) represents a novel species of a new genus within the order Thermotogales, for which the name Defluviitoga tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is SulfLac1(T) (=DSM 23805(T) =JCM 17210(T)).