Mass spectrometry identification of circulating alpha-1-B glycoprotein, increased in aged female C57BL/6 mice.
Mass spectrometry identification of circulating alpha-1-B glycoprotein, increased in aged female C57BL/6 mice.
复制标题
老年雌性 C57BL/6 小鼠中循环 α-1-B 糖蛋白的质谱鉴定有所增加。
DOI：
10.1016/j.bbagen.2006.06.020
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Cui,Zheng
中科院分区：
文献类型：
--
作者：
StehleJr,JohnR;Weeks,MarkE;Lin,Kai;Willingham,MarkC;Hicks,AmyM;Timms,JohnF;Cui,Zheng
本研究采用双向SDS-PAGE技术，对不同品系、性别和年龄小鼠的循环蛋白质谱进行了研究。在银染2-D凝胶上可见的蛋白质点中，我们鉴定了一组独特的7种与之相关的蛋白质，其水平在老年C57 BL/6雌性小鼠中持续升高。这组7种蛋白质在C57 BL/6雄性或任何年龄的任何性别的BALB/c小鼠中均不存在。当C57 BL/6雌性小鼠与BALB/c雄性小鼠杂交时，这些蛋白质的年龄相关性增加在F1后代中变得零星而非线性。挑取该蛋白质组的所有7个点，并在胰蛋白酶消化后通过质谱分析进行鉴定。结果表明，所有7个斑点都是α 1B-糖蛋白的不同亚型，具有不同程度的翻译后修饰，如磷酸化。这些结果表明，α 1B-糖蛋白在小鼠中的变化具有性别和年龄依赖性。
In this study, we surveyed the profiles of mouse circulating proteins by 2-dimensional SDS-PAGE in different strains, sexes and ages. Among visible protein spots on 2-D gels with silver-staining, we identified a unique set of 7 seemingly-related proteins whose levels were consistently elevated in older C57BL/6 female mice. This set of 7 proteins was absent in C57BL/6 males or in BALB/c mice of either sex of any age. When C57BL/6 female mice were crossed with BALB/c males, the age-related increase of these proteins became sporadic and not linear in the F1 offspring. All 7 spots of this protein group were picked and subjected to identification by mass spectrometric analysis after tryptic digestion. The results showed that all 7 spots were different isoforms of α1B-glycoprotein with different degrees of post-translational modifications, such as phosphorylation. These results suggest that α1B-glycoprotein changes in mice in a sex and age dependent manner.