IN VITRO Anticancer Activity and Physicochemical Properties of SOLANUM LYCOCARPUM Alkaloidic Extract Loaded in Natural Lipid-Based Nanoparticles
IN VITRO Anticancer Activity and Physicochemical Properties of SOLANUM LYCOCARPUM Alkaloidic Extract Loaded in Natural Lipid-Based Nanoparticles
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DOI：
10.1016/j.colcom.2018.11.001
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发表时间：
2019-01-01
影响因子：
4.5
通讯作者：
Marcato, P. D.
中科院分区：
文献类型：
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作者：
Carvalho, I. P. S.;Miranda, M. A.;Marcato, P. D.
膀胱癌（BC）是世界上第九大常见癌症。BC的治疗仍然是一个具有挑战性的目标。因此，筛选具有抗肿瘤活性的天然化合物如茄碱（SS）和茄边碱（SM）对BC治疗是有意义的。本工作旨在开发天然脂质纳米粒（NLN）作为S.富含SS和SM的石松果实生物碱提取物（AE），并评价其对膀胱癌细胞的抗肿瘤活性。通过LUMiSizer（R）测量，NLN-AE显示出56 nm的直径、低的多分散性指数，估计保质期为12个月。NLN-AE具有低的重结晶指数和高的SS（86%）和SM（89%）包封率。在36小时后验证了持续释放曲线。游离AE在膀胱癌细胞中表现出抗肿瘤活性。当AE被包裹在NLN中时，细胞活力下降5.4倍。因此，NLN-AE比游离AE具有更高的抗增殖活性。
Bladder cancer (BC) is the 9th most common cancer in the worldwide. BC's treatment is still a challenging goal. Thereby, the screening of natural compounds as solasonine (SS) and solamargine (SM) that exhibit antitumor activity is interesting to BC therapy. This work aimed to develop natural lipid-based nanoparticles (NLN) as carrier system of S. lycocarpum's fruits alkaloidic extract (AE), rich in SS and SM, and to evaluate its antitumor activity in bladder cancer cells. The NLN-AE exhibited 56 nm of diameter, low polydispersity index with shelf life estimated of 12 months, measured by LUMiSizer (R). NLN-AE showed low recrystallization index with high encapsulation efficiency of SS (86%) and SM (89%). A sustained release profile was verified after 36 h. The free AE exhibited antitumor activity in bladder cancer cells. The cell viability decreased 5.4 fold when AE was encapsulated in NLN. Thus, NLN-AE has higher anti-proliferative activity than the free AE.