GABAA receptor signaling in caudal periaqueductal gray regulates maternal aggression and maternal care in mice
GABAA receptor signaling in caudal periaqueductal gray regulates maternal aggression and maternal care in mice
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DOI：
10.1016/j.bbr.2010.05.001
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发表时间：
2010-12-01
影响因子：
2.7
通讯作者：
Gammie, Stephen C.
中科院分区：
文献类型：
--
作者：
Lee, Grace;Gammie, Stephen C.
母性攻击（母性防御）表现为哺乳期女性对入侵者的攻击，有助于保护后代。苯二氮卓类药物增强γ-氨基丁酸（GABA）（A）受体信号传导可提高母体攻击性，我们先前发现的间接证据（通过c-Fos免疫组织化学）表明，尾水管周围灰质（cPAG）和外侧隔（LS）可能是苯二氮卓类药物增加攻击性的部位。我们最近发现LS中的GABA（A）受体信号调节母体攻击，在这项研究中，我们测试了cPAG中的GABA（A）受体信号也调节这种行为的假设。使用GABA（A）受体拮抗剂荷包牡丹碱（3-9 ng）或GABA（A）受体阳性调节剂利血平（CDP）（一种苯二氮卓类药物）（2.5-20 μ g），对哺乳期小鼠进行cPAG定点注射。母亲的侵略，其他母亲的行为，和焦虑样措施（使用明暗箱），然后进行了检查。GABA（A）受体阳性调节剂没有增加攻击性，这可能是由于天花板效应。然而，cPAG中的8 ng和9 ng荷包牡丹碱显著降低了母体攻击性，而不改变其他母体行为或明暗箱性能，表明cPAG中的一些GABA（A）受体信号传导是完全母体攻击性表达所必需的。此外，7 ng荷包牡丹碱显著增加了幼崽的舔/梳理，并减少了亮-暗盒的亮和暗隔室之间的转换次数，而不影响攻击性。鉴于这些结果表明，拮抗cPAG中的GABA（A）受体剂量依赖性地促进后代理毛行为，同时损害攻击性，cPAG可能是哺乳期雌性决策（攻击性与其他行为）的关键部位。(C)2010 Elsevier B. V.保留所有权利。
Maternal aggression (maternal defense) is exhibited by lactating females towards intruders and contributes to the protection of offspring. Enhancement of Gamma-Aminobutyric acid (GABA)(A) receptor signaling by benzodiazepines elevates maternal aggression, and we previously found indirect evidence (via c-Fos immunohistochemistry) that caudal periaqueductal gray (cPAG) and lateral septum (LS) could be sites where benzodiazepines increase aggression. We recently found that GABA(A) receptor signaling in LS modulates maternal aggression, and in this study, we tested the hypothesis that GABA(A) receptor signaling in cPAG also regulates this behavior. Site-directed injections to cPAG were made in lactating mice using the GABA(A) receptor antagonist, bicuculline (3-9 ng) or the GABA(A) receptor positive modulator, chlordiazepoxide (CDP), a benzodiazepine (2.5-20 mu g). Maternal aggression, other maternal behaviors, and anxiety-like measures (using the light-dark box) were then examined. GABA(A) receptor positive modulator did not increase aggression, which could have resulted from a ceiling effect. However, 8 ng and 9 ng of bicuculline in cPAG significantly decreased maternal aggression without altering other maternal behaviors or light-dark box performance, suggesting some GABA(A) receptor signaling in cPAG is required for full maternal aggression expression. Additionally, 7 ng of bicuculline significantly increased licking/grooming of pups, and decreased the number of transitions between the light and dark compartments of the light-dark box without affecting aggression. Given these results indicating that antagonizing GABA(A) receptor in cPAG dose-dependently promotes offspring grooming behavior while impairing aggression, it is possible that the cPAG represents a key site for decision making (aggression versus other behaviors) in the lactating female. (C) 2010 Elsevier B.V. All rights reserved.