An inexpensive system for imaging the contents of multi-well
An inexpensive system for imaging the contents of multi-well
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DOI：
10.1107/s2053230x18016515
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发表时间：
2018-12-01
影响因子：
0.9
通讯作者：
Bohm, Andrew
中科院分区：
文献类型：
--
作者：
Bohm, Andrew
一个廉价的系统，自动成像的内容物的12-，24-和96-孔板已建成。xyz阶段是由轻型计算机数控木雕/雕刻机的部件构成的，基于Arduino的电路板是有线的，这样它就可以通过显微镜安装的数码相机触发静止图像或电影。平移台提供样品在x方向160 mm、y方向100 mm和z方向40 mm的体积内的可再现三维移动。Python脚本生成G代码命令文件，该文件扫描板并收集每个样品的一系列z堆叠图像。第二个Python脚本自动计算具有数字增强景深的图像。该成像系统目前用于促进蛋白质晶体的筛选，但它可以用于自动成像多孔板中的许多其他类型的样品。
An inexpensive system for automated imaging of the contents of 12-, 24- and 96-well plates has been built. The xyz stage is constructed from parts from a light-duty computer numerical control wood-carving/engraving machine, and the Arduino-based board was wired so that it can trigger still images or movies though a microscope-mounted digital camera. The translation stage provides reproducible three-dimensional movement of the sample over a volume of 160 mm in x, 100 mm in y and 40 mm in z. A Python script generates the G-code command file that scans the plate and collects a series of z-stacked images of each sample. A second Python script automates the calculation of images with a digitally enhanced depth of field. The imaging system is currently being used to facilitate screening for protein crystals, but it could be used to automate the imaging of many other types of samples in multi-well plates.