Intradermal vaccination of dendritic cell-derived exosomes is superior to a subcutaneous one in the induction of antitumor immunity
Intradermal vaccination of dendritic cell-derived exosomes is superior to a subcutaneous one in the induction of antitumor immunity
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DOI：
10.1089/cbr.2006.21.146
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发表时间：
2006-04-01
影响因子：
3.4
通讯作者：
Xiang, Jim
中科院分区：
文献类型：
--
作者：
Hao, Siguo;Ye, Zhenmin;Xiang, Jim
由于树突状细胞（DC）衍生的外泌体（EXO）含有许多参与诱导免疫应答的重要DC分子，因此基于EXO的疫苗已广泛用于在不同动物肿瘤模型中诱导抗肿瘤免疫。然而，目前尚不清楚哪种给药途径能诱导更有效的自身肿瘤免疫反应。在这项研究中，我们比较了两种常见给药途径，皮下（s.c）和皮内（i.d）给药的EXO疫苗的抗肿瘤免疫。我们的数据显示，与s.c.给药相比，W. EXO给药导致更多EXO吸收的DC迁移到引流淋巴结的t细胞区域。有趣的是，与s. c.给药相比，d. EXO给药也导致体内卵清蛋白（OVA）特异性CD8(+) t细胞增殖和CD8(+) CTL效应反应增强。同样，与s.c.疫苗相比，i.d疫苗在动物肿瘤模型中诱导了更强的抗肿瘤免疫。因此，在设计EXO型疫苗时，应考虑到id . EXO疫苗优于sc疫苗。
Because dendritic cell (DC)-derived exosomes (EXO) harbor many important DC molecules involved in inducing immune responses, EXO-based vaccines have been extensively used to induce antitumor immunity in different animal tumor models. However, it is not clear which route of EXO administration can induce more efficient autitumor immune responses. In this study, we compared the antitumor immunity derived from EXO vaccine by way of the two common administration routes, the subcutaneous (s.c.) and the intradermal (i.d.) adminstrations. Our data showed that the W. EXO administration resulted in more EXO-absorbed DC migrating into the T-cell areas of draining lymph nodes than the s.c. administration. Interestingly, the i.d. EXO administration also resulted in an enhanced ovalbumin (OVA)-specific CD8(+) T-cell proliferation and CD8(+) CTL effector responses in vivo, compared to the s. c. administration. Similarly, compared to the s.c. vaccination, the i.d. vaccination induced stronger antitumor immunity in the animal tumor model. Therefore, the i.d. EXO vaccination is superior to the s.c. one and should be considered when EXO-based vaccine is designed.