Microvessel Density Is Associated with VEGF and α-SMA Expression in Different Regions of Human Gastrointestinal Carcinomas.
Microvessel Density Is Associated with VEGF and α-SMA Expression in Different Regions of Human Gastrointestinal Carcinomas.
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DOI：
10.3390/cancers3033405
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发表时间：
2011-08-31
期刊：
影响因子：
5.2
通讯作者：
Abreu C
中科院分区：
文献类型：
--
作者：
Tonino P;Abreu C
已知肿瘤血管生成受宿主和肿瘤细胞分泌的生长因子调节。尽管肿瘤血管和血管生成异质性在实体瘤中的重要性，但很少有研究比较人类癌症不同区域的血管。肿瘤不同部位的血管在肿瘤血管生成中可能具有形态功能意义。因此，本研究探讨了胃肠道癌（GITC）肿瘤中心区（CT）、周围区（P）和转移区（M）微血管密度（MVD）与血管内皮生长因子（VEGF）表达和α-平滑肌肌动蛋白（α-SMA）表达的关系，以及MVD与临床病理因素的关系。对27例患者手术切除的CT、P、M标本进行FVIII、VEGF和α-SMA免疫组化检测。CT、P、M区MVD与GITC无显著性差异。VEGF阳性组MVD显著高于VEGF阴性组（CT，p = 0.034; P，p = 0.030; M，p = 0.032）。与M区相比，CT和P区的MVD作为α-SMA表达的函数也显著更高（p = 0.0008）。总之，MVD与VEGF和α-SMA表达的相关性可能表明GITC不同区域中新生血管和原有血管的数量增加，这些血管均匀或部分被周细胞覆盖，提示MVD和VEGF不仅是肿瘤血管的重要参数，而且血管成熟是胃肠道肿瘤血管生成调节的关键因素，可能是血管治疗的靶点。
Tumor angiogenesis is known to be regulated by growth factors secreted by host and tumor cells. Despite the importance of tumor vasculature and angiogenic heterogeneity in solid tumors, few studies have compared the vasculature in different regions of human cancer. Blood vessels from different regions of carcinomas might have morphofunctional implications in tumor angiogenesis. In the present study, therefore, we have examined the relationship between microvascular density (MVD) and vascular endothelial growth factor (VEGF) expression and alpha smooth muscle actin (α-SMA) expression in the center of the tumor (CT), periphery (P) and metastasis (M) regions from gastrointestinal carcinomas (GITC), as well as the association of MVD with clinicopathological factors. Surgically resected specimens corresponding to the CT, P and M from 27 patients were examined for FVIII, VEGF and α-SMA by immunohistochemistry. The MVD was not significantly different in the CT, P and M regions from GITC. The MVD in the VEGF positive group was significantly higher than in the VEGF negative group (CT, p = 0.034; P, p = 0.030; M, p = 0.032). The MVD as a function of α-SMA expression was also significantly higher in the CT and P region compared to the M region (p = 0.0008). In conclusion, the MVD association with VEGF and α-SMA expression, might indicate an increase of the number of neoformed and preexisting blood vessels uniformly or partially covered by pericytes in different regions of GITC, suggesting that not only MVD and VEGF are important parameters to the tumor vasculature, but also blood vessels maturation is a crucial factor for gastrointestinal tumor angiogenesis regulation and possible target of vascular therapy.