Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway
Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway
复制标题
微波暴露通过过度激活 NMDA 受体信号通路损害大鼠海马和 PC12 细胞的突触可塑性
DOI：
10.3967/bes2015.002
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发表时间：
2015-01-01
影响因子：
3.5
通讯作者：
Peng Rui Yun
中科院分区：
文献类型：
--
作者：
Xiong Lu;Sun Cheng Feng;Peng Rui Yun
目的研究微波辐射对N-甲基-D-天冬氨酸受体（NMDAR）信号通路的影响，探讨NMDAR信号通路在突触可塑性损伤中的作用。通过H&E染色和透射电镜观察海马结构。用30 mW/cm 2微波照射PC 12细胞5 min，扫描电镜和原子力显微镜观察突触形态。检测氨基酸类神经递质的释放和钙内流。结果微波辐射可引起大鼠海马结构和PC 12细胞的损伤，尤其是突触的结构和数量的改变。谷氨酸和γ-氨基丁酸神经递质的比例增加，细胞内钙水平升高。结论30 mW/cm 2微波辐射可引起突触结构、氨基酸神经递质释放和钙内流的改变。NMDAR信号分子与突触可塑性受损密切相关。
Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor (NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment.Methods 48 male Wistar rats were exposed to 30 mW/cm(2) microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 mW/cm(2) microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated.Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits (NR1, NR2A, and NR2B) and related signaling molecules (Ca2+/calmodulin-dependent kinase II gamma and phosphorylated cAMP-response element binding protein) were examined.Conclusion 30 mW/cm(2) microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity.