Detection of Epstein-Barr virus in the brain by the polymerase chain reaction.
Detection of Epstein-Barr virus in the brain by the polymerase chain reaction.
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通过聚合酶链式反应检测大脑中的 Epstein-Barr 病毒。
DOI：
10.1002/ana.410320210
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发表时间：
1992
影响因子：
11.2
通讯作者：
Miller,G
中科院分区：
文献类型：
--
作者：
Pedneault,L;Katz,BZ;Miller,G
Epstein-巴尔病毒（EBV）与多种中枢神经系统综合征有关。在少数研究充分的患者中，通过病毒分离检测到EBV或通过Southern杂交分析发现EBV DNA。使用聚合酶链反应，我们评估了24例患者的脑活检标本中是否存在EBV基因组。在18名临床或血清学检测怀疑脑内存在病毒的患者的脑标本中发现了EBV DNA。6例患者患有获得性免疫缺陷综合征; 2例为肾移植受者。4例其他疱疹病毒引起脑炎患者和1例代谢性脑病患者的脑标本经聚合酶链反应检测，EBV DNA呈阴性。这些发现表明需要评估EBV在不同神经系统综合征中的作用，特别是那些发生在免疫缺陷宿主中的神经系统综合征。
Epstein‐Barr virus (EBV) has been implicated in a variety of central nervous system syndromes. In a few well‐studied patients, EBV has been detected by viral isolation or EBV DNA has been found by Southern hybridization analysis. Using polymerase chain reaction, we evaluated brain biopsy specimens from 24 patients for the presence of EBV genomes. EBV DNA was found in brain specimens from 18 patients in whom presence of the virus in the brain was suspected clinically or on the basis of serological tests. Six patients had acquired immunodeficiency syndrome; 2 were kidney transplant recipients. Brain specimens from 4 patients with encephalitis due to other herpes group viruses and from a patient with metabolic encephalopathy were negative for EBV DNA as determined by polymerase chain reaction. The findings indicate a need to evaluate the role of EBV in diverse neurological syndromes, especially those occurring in immunodeficient hosts.