Photo-Cross-Linked Scaffold with Kartogenin-Encapsulated Nanoparticles for Cartilage Regeneration
Photo-Cross-Linked Scaffold with Kartogenin-Encapsulated Nanoparticles for Cartilage Regeneration
复制标题
用于软骨再生的带有 Kartogenin 封装纳米颗粒的光交联支架
DOI：
10.1021/acsnano.5b06663
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发表时间：
2016-01-01
期刊：
影响因子：
17.1
通讯作者：
Gu, Zhen
中科院分区：
文献类型：
--
作者：
Shi, Dongquan;Xu, Xingquan;Gu, Zhen
软骨是一种无血管和无血管组织，其再生常常因其缺乏产生足够愈合反应的先天能力而受到损害。 Kartogenin (KGN) 是一种小分子化合物，可以诱导骨髓来源的间充质干细胞 (BMSC) 转化为软骨细胞。先前的体外研究表明，kartogenin 对滑膜来源的间充质干细胞 (SMSC) 也具有软骨形成作用。在此，我们展示了使用一种创新的一步技术将紫外线反应性、快速交联的支架与负载卡托根元的纳米颗粒集成的效果。体内研究表明其在细胞归巢方面的潜在作用，特别是在无需细胞移植的情况下招募宿主的内源细胞（包括 BMSC 和 SMSC）。值得注意的是，根据组织学测试、特异性标记物分析和生物力学测试，再生组织接近天然透明软骨。这种创新的 KGN 释放系统使软骨形成高效且持久。
The regeneration of cartilage, an aneural and avascular tissue, is often compromised by its lack of innate abilities to mount a sufficient healing response. Kartogenin (KGN), a small molecular compound, can induce bone marrow-derived mesenchymal stem cells (BMSCs) into chondrocytes. The previous in vitro study showed that kartogenin also had a chondrogenesis effect on synovium derived mesenchymal stein cells (SMSCs). Herein, we present the effect of an ultraviolet-reactive, rapidly cross-linkable scaffold integrated with kartogenin-loaded nanoparticles using an innovational one-step technology. In vivo studies showed its potential role for cell homing, especially for recruiting the host's endogenous cells, including BMSCs and SMSCs, without cell transplantation. Of note, the regenerated tissues were close to the natural hyaline cartilage based on the histological tests, specific markers analysis, and biomechanical tests. This innovative KGN release system makes the chondrogenesis efficient and persistent.