Resuscitation of functional bacterial community for enhancing biodegradation of phenol under high salinity conditions based on Rpf
Resuscitation of functional bacterial community for enhancing biodegradation of phenol under high salinity conditions based on Rpf
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基于Rpf的功能性细菌群落复苏以增强高盐条件下苯酚的生物降解
DOI：
10.1016/j.biortech.2018.04.048
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发表时间：
2018
影响因子：
11.4
通讯作者：
Sun Faqian
中科院分区：
文献类型：
--
作者：
Su Xiaomei;Wang Yuyang;Xue Binbing;Zhang Yunge;Mei Rongwu;Zhang Yu;Hashmi Muhammad Zaffar;Lin Hongjun;Chen Jianrong;Sun Faqian
本研究假设功能菌群的关键降解菌在高盐酚条件下容易进入活的但不可培养的（VBNC）状态，复苏促进因子（Rpf）可以强化这些降解菌以获得更好的性能。在此基础上，利用Rpf提高活性污泥中功能菌群的耐盐降酚能力。结果表明，Rpf能加速污泥驯化过程中的启动过程，显著提高耐盐苯酚降解能力。高通量测序结果表明，Rpf的复苏和刺激功能主要与放线菌门中的棒状杆菌属和拟杆菌门中的嗜蛋白菌属和石化单胞菌属有关。这些关键功能种群在高盐条件下具有更好的苯酚降解能力。研究表明，Rpf是一种很有前途的含盐含酚废水生物处理添加剂。
This study assumed that key degraders of functional bacterial community were prone to enter into the viable but non-culturable (VBNC) state under high saline phenolic conditions, and resuscitation-promoting factor (Rpf) could strengthen these degraders for better performances. Based on these assumptions, Rpf was used to enhance salt-tolerant phenol-degrading capability of functional populations in activated sludge. Results suggested that Rpf accelerated the start-up process during sludge domestication, and significantly enhanced salt-tolerant phenol-degrading capability. High-throughput sequencing showed that the resuscitation and stimulation functions of Rpf linked mainly to the genusCorynebacteriumwithin the phylumActinobacteria, and the generaProteiniphilumandPetrimonaswithin the phylumBacteroidete. These key functional populations contributed to better phenol-degrading capabilities under high salinity conditions. This study indicated that Rpf is a promising additive for improving biological treatment performance of saline phenolic wastewater.