A novel testis-specific Na+/H+ exchanger is involved in sperm motility and fertility.
A novel testis-specific Na+/H+ exchanger is involved in sperm motility and fertility.
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DOI：
10.2741/e115
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Liu, Yi-Xun
中科院分区：
文献类型：
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作者：
Liu, Tao;Huang, Jun-Cheng;Liu, Yi-Xun
钠氢交换器作为调节细胞内pH的通道，可能是精子获能和运动的重要调节器。在精子中已鉴定出该家族的三个成员。本研究克隆了一种新的睾丸特异性钠氢交换蛋白mtsNHE。mtsNHE定位于精子鞭毛的主片段上，包含12个预测的跨膜区，羧基端无胞质片段。亲水区在钠氢交换器家族成员中普遍存在。跨膜区多克隆抗体显著降低精子活力、顶体反应和体外受精率。将抗体注入精子液中，可降低细胞内pH值和钙离子浓度。肌肉注射mtsNHE特异性基因疫苗可显著降低雌性小鼠的生育率。考虑到其特异性表达和参与生育调节，mtsNHE可能是开发新的男性避孕药的潜在靶分子。
Sodium-hydrogen exchanger as a channel for regulation of intracellular pH might be a crucial modulator of sperm capacitation and motility. Three members of this family have been identified in spermatozoa. A novel protein testis-specific sodium-hydrogen exchanger named mtsNHE was cloned in the present study. The mtsNHE localizing on principle piece of sperm flagellum contained 12 predicted transmembrane regions without cytoplasmic fragment at carboxyl terminus. Hydrophilic region was common in the sodium-hydrogen exchanger family members. Polyclonal antibodies to trans-membrane region significantly reduced sperm motility, acrosome reaction and ratio of in vitro fertilization. By in-pouring the antibodies in sperm solution, intracellular pH and calcium concentration were decreased. Muscle injection of female mice with the specific gene vaccine of mtsNHE, significantly stepped down fertility rate. Considering its specific expression and involvement in the regulation of fertility, the mtsNHE might be a potential target molecule for developing a new male contraceptive.