Effects of sediment storage conditions on pigment analyses
Effects of sediment storage conditions on pigment analyses
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DOI：
10.4319/lom.2005.3.477
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发表时间：
2005-10
期刊：
影响因子：
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通讯作者：
Nina S. Reuss;D. Conley
中科院分区：
文献类型：
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作者：
Nina S. Reuss;D. Conley
在过去几十年中，沉积物色素作为水柱和底栖生物过程的指示剂已得到广泛应用。然而，不同的储存和处理条件对沉积物色素分析的准确性的影响还没有得到充分的评价。这可能会使研究之间的比较变得困难，在最坏的情况下，会对一些研究的有效性提出质疑。尽管沉积物色素研究通常依赖于单个色素随深度的相对变化，但储存和处理的影响是任何调查中的关键考虑因素。根据对一些不同方案的测试，本研究建议，所有沉积物样品应在取样后尽快在-20 °C或更低温度下冷冻，并在此温度下储存，无需额外处理（例如，冷冻干燥）直到刚好在分析颜料之前。在低于-20 °C的温度下冷冻或用氮气冲洗并不能改善保存。我们的结果表明，如果根据其他替代分析的要求，原始沉积物可以在3°C下储存长达6个月，而冻干沉积物应始终冷冻储存。此外，强烈建议使用内标物（例如β-apo-8-carotenal）对色素浓度进行归一化，因为它可显著降低运行之间的变异性。
Sediment pigments as an indicator of water column and benthic processes have become widely used during the last decades. However, the effect of different storage and handling conditions on the accuracy of sediment pigment analyses has not been adequately evaluated. This can potentially make comparisons between studies difficult and, at worst, call into question the validity of some studies. Even though sediment pigment studies often rely on relative changes of individual pigments with depth, the effect of storage and handling is a critical consideration in any investigation. Based on testing of a number of different protocols, this study recommends that all sediment samples should be frozen at −20°C or colder as soon as possible after sampling and stored at this temperature without additional treatment (e.g., freeze‐drying) until just before analysis of pigments. Freezing at a lower temperature than −20°C or flushing with nitrogen gas did not improve preservation. Our results showed that, if dictated by other proxy analyses, raw sediment can be stored at 3°C for up to 6 months, whereas freeze‐dried sediment should always be stored frozen. In addition, the use of an internal standard (for example, β‐apo‐8‐carotenal) for normalizing pigment concentrations is highly recommended as it significantly reduces variability between runs.