Investigations of Coherent Vibrational Oscillations in Myoglobin
Investigations of Coherent Vibrational Oscillations in Myoglobin
复制标题
肌红蛋白相干振动的研究
DOI：
10.1021/jp993617f
复制
发表时间：
2000
影响因子：
2.9
通讯作者：
P. Champion
中科院分区：
文献类型：
--
作者：
F. Rosca;Anand Kumar;Xiong Ye;T. Sjodin;A. Demidov;P. Champion
将飞秒相干光谱(FCS)技术应用于肌红蛋白的研究。研究了脱氧肌红蛋白(Mb)的光稳定性样品和一氧化氮加合物(MbNO)的光化学活性样品。泵浦引起的两个样品的探测透过率的变化表现出相干振荡，当转换到频域时，与脱氧Mb的共振拉曼光谱一致。这表明，与光反应样品(MBNO)相关的相干是由反应物和产物态势能面之间的交叉区(S)出现的快速变化力引起的。分析了与血红素和蛋白质之间唯一的共价键有关的Fe−His振动的相对相位和幅度随样品状态和泵浦/探测载波频率的变化。对于Mb中的“场驱动”相干和“反应”相干，相位对载波频率的依赖关系被发现是显著不同的。
The technique of femtosecond coherence spectroscopy (FCS) is applied to the heme protein myoglobin. Photostable samples of deoxy myoglobin (Mb) and photochemically active samples of the nitric oxide adduct (MbNO) are investigated. The pump-induced change in the probe transmittance for both samples displays coherent oscillations that, when transformed into the frequency domain, are in agreement with the resonance Raman spectrum of deoxy Mb. This indicates that the coherences associated with the photoreactive sample (MbNO) arise from the rapidly changing forces appearing in the crossing region(s) between the reactant and product state potential energy surfaces. The relative phase and amplitude of the Fe−His vibration, associated with the sole covalent linkage between the heme and the protein, are analyzed as a function of sample state and pump/probe carrier frequency. The dependence of the phase on carrier frequency is found to be significantly different for the “field driven” coherence in Mb and the “react...