Expression of human DEC-205 (CD205) multilectin receptor on leukocytes
Expression of human DEC-205 (CD205) multilectin receptor on leukocytes
复制标题
DOI：
10.1093/intimm/dxl022
复制
发表时间：
2006-06-01
影响因子：
4.4
通讯作者：
Hart, Derek N. J.
中科院分区：
文献类型：
--
作者：
Kato, Masato;McDonald, Kylie J.;Hart, Derek N. J.
DEC-205 （CD205）属于巨噬细胞c型凝集素内吞受体甘露糖受体家族，是树突状细胞（DC）的抗原摄取/加工受体。为了研究DEC-205在人白细胞中的组织分布，我们制备了一系列抗人DEC-205单克隆抗体（MMRI-5、6和7），它们识别c型凝集素样结构域1和2内的表位，MMRI-7免疫沉淀出一个类似200 kDa的单带，通过质谱鉴定为DEC-205。MMRI-7和另一种识别富含半胱氨酸和纤维连接蛋白II型结构域表位的DEC-205 mAb （MG38）用于检测DEC-205在人白细胞中的表达。与小鼠DC上主要表达的DEC-205不同，流式细胞术检测到人DEC-205在髓系血液DC和单核细胞中表达水平相对较高，在B淋巴细胞中表达水平中等，在NK细胞、浆细胞样血液DC和T淋巴细胞中表达水平较低。mmri - 7f （ab′）(2)也同样标记单核细胞、B淋巴细胞和NK细胞，排除了单抗与Fc γ r非特异性结合的反应性。扁桃体单核细胞在白细胞上显示出相似的DEC-205染色分布。特异的半定量免疫沉淀/western blot和定量逆转录- pcr分析证实，这些白细胞群表达decc -205蛋白和同源mRNA。因此，与先前基于小鼠DEC-205组织定位研究的假设相比，人类DEC-205在更多的白细胞群中表达。在人体内广泛的DEC-205组织表达与临床DC靶向策略和DEC-205功能研究有关。
DEC-205 (CD205) belongs to the macrophage mannose receptor family of C-type lectin endocytic receptors and behaves as an antigen uptake/processing receptor for dendritic cells (DC). To investigate DEC-205 tissue distribution in human leukocytes, we generated a series of anti-human DEC-205 monoclonal antibodies (MMRI-5, 6 and 7), which recognized epitopes within the C-type lectin-like domains 1 and 2, and the MMRI-7 immunoprecipitated a single similar to 200 kDa band, identified as DEC-205 by mass spectrometry. MMRI-7 and another DEC-205 mAb (MG38), which recognized the epitope within the DEC-205 cysteine-rich and fibronectin type II domain, were used to examine DEC-205 expression by human leukocytes. Unlike mouse DEC-205, which is reported to have predominant expression on DC, human DEC-205 was detected by flow cytometry at relatively high levels on myeloid blood DC and monocytes, at moderate levels on B lymphocytes and at low levels on NK cells, plasmacytoid blood DC and T lymphocytes. MMRI-7 F(ab')(2) also labeled monocytes, B lymphocytes and NK cells similarly excluding reactivity due to non-specific binding of the mAb to Fc gamma R. Tonsil mononuclear cells showed a similar distribution of DEC-205 staining on the leukocytes. DEC-205-specific semiquantitative immunoprecipitation/western blot and quantitative reverse transcriptase-PCR analysis established that these leukocyte populations expressed DEC-205 protein and the cognate mRNA. Thus, human DEC-205 is expressed on more leukocyte populations than that were previously assumed based on mouse DEC-205 tissue localization studies. The broader DEC-205 tissue expression in man is relevant to clinical DC targeting strategies and DEC-205 functional studies.