Molecular epidemiologic analysis of group A rotaviruses in adults and children with diarrhea in Wuhan city, China, 2000–2006
Molecular epidemiologic analysis of group A rotaviruses in adults and children with diarrhea in Wuhan city, China, 2000–2006
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DOI：
10.1007/s00705-006-0904-y
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发表时间：
2007-01
影响因子：
2.7
通讯作者：
Yi Wang;N. Kobayashi;D. Zhou;Zhan-Qiu Yang;Xuan Zhou;J. Peng;Ze-rong Zhu;Dongmei Zhao;Manqing Liu;J. Gong
中科院分区：
文献类型：
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作者：
Yi Wang;N. Kobayashi;D. Zhou;Zhan-Qiu Yang;Xuan Zhou;J. Peng;Ze-rong Zhu;Dongmei Zhao;Manqing Liu;J. Gong
为比较引起儿童和成人腹泻的A组轮状病毒的流行病学特征和基因特征，于2000年12月至2006年5月在武汉市中国进行了调查。对8家医院3839份腹泻患者粪便标本进行分析。成人和儿童轮状病毒腹泻具有冬季季节性，轮状病毒总阳性率分别为9.0%和23.9%。在整个研究期间，G3型轮状病毒在成人和儿童中都是最常见的G血清型(检出率分别为86.2%和87.8%)，并且主要与VP4型[8]、VP6型II(亚组II)和NSP4B型有关。G3型轮状病毒分为8种电泳型，其中7种在成人和儿童标本中都发现。不同年份、不同医院的G3轮状病毒成人株和儿童株(分别为6株和4株)的VP7基因序列具有极高的同源性(99-100%)，与亚洲报道的少数G3轮状病毒株也有很高的同源性。然而，迄今为止报道的大多数人和动物G3轮状病毒的序列同源性较低(82-96%)，包括一些中国株。结果表明，武汉市中国地区轮状病毒在成人和儿童中的流行特征和基因特征相似，提示G3轮状病毒可能起源于同一轮状病毒，在儿童和成人中流行。L169株VP7基因属于全球广泛流行的G9轮状病毒的主要谱系，但不同于中国以前报道的G9轮状病毒。R479株VP7基因与大多数G4人类轮状病毒不同，表现出罕见的双亚群特异性，即I+II。R479 VP6基因在系统发育上不属于I和II亚群轮状病毒的主要聚集区，但与猪轮状病毒和以印度东部分离的RMC321株为代表的一些罕见的人类轮状病毒的VP7基因有亲缘关系。
To compare epidemiologic features and genetic characteristics of group A rotaviruses causing diarrhea in children and adults, a survey was conducted in Wuhan, China, during the period of Dec. 2000–May 2006. A total of 3839 stool specimens from diarrheal patients from eight hospitals were analyzed. Winter seasonality was observed for rotavirus diarrhea in both adults and children, showing overall rotavirus-positive rates of 9.0 and 23.9%, respectively. Throughout the study period, G3 was the most frequent G serotype in both adults and children (detection rates 86.2 and 87.8%, respectively), and was mostly associated with VP4 genotype P[8], VP 6 genotype II (subgroup II), and NSP4 genotype B. G3 rotaviruses were differentiated into eight electropherotypes, among which seven types were found in specimens from both adults and children. VP7 gene sequences of G3 rotaviruses from adults and children (6 and 4 strains, respectively), detected in different years and different hospitals, showed extremely high sequence identities (99–100%) to each other and to a few G3 rotavirus strains reported in Asia. However, lower sequence identities (82–96%) were observed to most of the human and animal G3 rotaviruses reported so far, including some Chinese strains. These findings indicate that in Wuhan, China, epidemic and genetic features of rotaviruses are similar in adults and children, and it has been suggested that G3 rotaviruses that might have originated from the same rotavirus were circulating among children and adults as prevailing viruses.In this study, two rotavirus strains, G9P[8] strain L169, derived from an adult, and G4P[6] strain R479, derived from a child, were isolated and genetically analyzed. The VP7 gene of L169 belongs to a major lineage of G9 rotaviruses that are globally widespread, but is distinct from G9 rotaviruses reported previously in China. The strain R479 had a VP7 gene which was divergent from most G4 human rotaviruses and showed an unusual dual subgroup specificity, I + II. The R479 VP6 gene does not belong to the main clusters of subgroup I and II rotaviruses phylogenetically, but is related to those of the porcine rotaviruses and some unusual human rotaviruses represented by the RMC321 strain isolated in eastern India.