Aging and cholinergic responses in bovine trachealis muscle.
Aging and cholinergic responses in bovine trachealis muscle.
复制标题
牛气管肌的衰老和胆碱能反应。
DOI：
10.1111/j.1476-5381.1988.tb11480.x
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发表时间：
1988
影响因子：
7.3
通讯作者：
Douglas,JS
中科院分区：
文献类型：
--
作者：
Wills,M;Douglas,JS
1.作为老化的结果，毒蕈碱激动剂对牛气管平滑肌的相对效力不变，卡巴胆碱大于氧震颤素大于毒蕈碱大于毛果芸香碱大于McNeil A-343。2.在老化过程中，卡巴胆碱，oxotremorine，麦克尼尔A-343和毛果芸香碱的效力，但不是毒蕈碱，减少。3.最大诱导紧张局势的所有研究的代理人作为年龄的结果减少。4.不可逆的拮抗作用与苄胆碱芥子气表明，激动剂的效力显着降低老化过程中。5.对于所研究的每种激动剂，成熟奶牛与未成熟奶牛气管组织中EC 50的估计受体占有率显著更高。6.完全激动剂（卡巴胆碱，oxotremorine和乙酰甲胆碱）的解离常数随着成熟而降低，而部分激动剂（麦克尼尔A-343，毛果芸香碱）则匡威。7.我们的结论是有显着的变化，在老化过程中的性能和耦合的毒蕈碱受体。这些变化可能导致体内观察到的气道反应性降低。
1. The relative potencies of muscarinic agonists on bovine tracheal smooth muscle were unchanged as a consequence of aging and were carbachol greater than oxotremorine greater than muscarine greater than pilocarpine greater than McNeil A-343. 2. During aging, the potencies of carbachol, oxotremorine, McNeil A-343 and pilocarpine, but not muscarine, were reduced. 3. Maximal induced tensions to all the agents studied were reduced as a consequence of age. 4. Irreversible antagonism with benzilylcholine mustard showed that agonist efficacy was significantly reduced during aging. 5. Estimated receptor occupancy at the EC50 was significantly greater in tracheal tissues from the mature versus immature cows for every agonist studied. 6. The dissociation constants for full agonists (carbachol, oxotremorine and methacholine) were decreased with maturation while the converse was observed with partial agonists (McNeil A-343, pilocarpine). 7. We conclude that there are significant changes in the properties and coupling of muscarinic receptors during aging. These changes may contribute to the reduced airway reactivity seen in vivo.