The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection
The up-regulation of IL-6 in DRG and spinal dorsal horn contributes to neuropathic pain following L5 ventral root transection
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DRG 和脊髓背角 IL-6 的上调导致 L5 腹根横断后的神经性疼痛
DOI：
10.1016/j.expneurol.2012.12.007
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发表时间：
2013-03
影响因子：
5.3
通讯作者：
Liu, Xian-Guo
中科院分区：
文献类型：
--
作者：
Chen, Feng-Ying;Li, Yong-Yong;Zang, Ying;Liu, Xian-Guo
我们的前期工作表明，促炎细胞因子肿瘤坏死因子-α（TNF-α）在L5-VRT引起的神经病理性疼痛中起重要作用。在目前的工作中，我们评估的作用，白细胞介素-6（IL-6），另一个关键的炎症细胞因子，在L5-VRT模型。我们发现，IL-6在同侧L4和L5背根神经节（DRG）神经元和双侧腰髓后L5-VRT上调。双重免疫荧光染色显示，在DRG中，IL-6的免疫反应性（IR）增加几乎局限于神经元细胞，而在脊髓背角中，IL-6-IR在胶质细胞（星形胶质细胞和小胶质细胞）和神经元中均上调。鞘内注射IL-6中和抗体显著延迟了L5-VRT后双侧后爪机械性异常性疼痛的诱导。此外，通过腹膜内沙利度胺抑制TNF-α合成可防止L5-VRT后机械性异常性疼痛和DRG中IL-6的上调。提示运动纤维损伤后传入神经元和脊髓中IL-6的表达增加与神经病理性疼痛的发生有关，TNF-α参与了IL-6的上调。
Our previous works have shown that pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) plays an important role in neuropathic pain produced by lumber 5 ventral root transection (L5-VRT). In the present work we evaluate the role of interleukin-6 (IL-6), another key inflammatory cytokine, in the L5-VRT model. We found that IL-6 was up-regulated in the ipsilateral L4 and L5 dorsal root ganglian (DRG) neurons and in bilateral lumbar spinal cord following L5-VRT. Double immunofluorescence stainings revealed that in DRGs the increased immunoreactivity (IR) of IL-6 was almost restricted in neuronal cells, while in the spinal dorsal horn IL-6-IR up-regulated in both glial cells (astrocyte and microglia) and neurons. Intrathecal administration of IL-6 neutralizing antibody significantly delayed the induction of mechanical allodynia in bilateral hindpaws after L5-VRT. Furthermore, inhibition of TNF-α synthesis by intraperitoneal thalidomide prevented both mechanical allodynia and the up-regulation of IL-6 in DRGs following L5-VRT. These data suggested that the increased IL-6 in afferent neurons and spinal cord contribute to the development of neuropathic pain following motor fiber injury, and that TNF-α is responsible for the up-regulation of IL-6.
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DOI：
10.1523/jneurosci.19-11-04305.1999
发表时间：
1999-06
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
J. Zhong;I. Dietzel;P. Wahle;M. Kopf;R. Heumann
通讯作者：
J. Zhong;I. Dietzel;P. Wahle;M. Kopf;R. Heumann
DOI：
10.1523/jneurosci.22-17-07746.2002
发表时间：
2002-09
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
Gang Wu;M. Ringkamp;B. Murinson;E. Pogatzki;T. Hartke;Himali M. Weerahandi;J. Campbell;J. Griffin-J.-Gr
通讯作者：
Gang Wu;M. Ringkamp;B. Murinson;E. Pogatzki;T. Hartke;Himali M. Weerahandi;J. Campbell;J. Griffin-J.-Gr
影响因子：
5.8
作者：
Vanden Berghe, W;Vermeulen, L;Haegeman, G
通讯作者：
Haegeman, G
影响因子：
4.2
作者：
Wieseler-Frank, J;Maier, SF;Watkins, LR
通讯作者：
Watkins, LR
影响因子：
3.6
作者：
Lee, Kyung-Min;Jeon, Sang-Min;Cho, Hee-Jung
通讯作者：
Cho, Hee-Jung