DISTRIBUTION OF GLUCAGONLIKE PEPTIDE-I (GLP-I), GLUCAGON, AND GLICENTIN IN THE RAT-BRAIN - AN IMMUNOCYTOCHEMICAL STUDY
DISTRIBUTION OF GLUCAGONLIKE PEPTIDE-I (GLP-I), GLUCAGON, AND GLICENTIN IN THE RAT-BRAIN - AN IMMUNOCYTOCHEMICAL STUDY
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DOI：
10.1002/cne.902710405
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发表时间：
1988-05-22
影响因子：
2.5
通讯作者：
LUND, PK
中科院分区：
文献类型：
--
作者：
JIN, SLC;HAN, VKM;LUND, PK
虽然胰高血糖素样免疫反应物（GLIs）存在于几种哺乳动物的中枢神经系统中，但其与胰高血糖素原的结构关系尚不明确，其精确的解剖分布也未得到广泛研究。为了进一步了解脑GLIs的结构和生物学意义，利用免疫细胞化学方法，利用亲和素-生物素-过氧化物酶方法，绘制了秋水仙碱处理大鼠大脑中胰高血糖素前-胰高血糖素样肽I （GLP - I）、胰高血糖素和胰甘肽素三种不同抗原决定因子的解剖分布。神经细胞体对GLP - 1、胰高血糖素和glicentin特异性抗血清有免疫反应，仅在延髓尾部发现。在尾侧髓质内，免疫染色的细胞体分布在约0.55 mm至0.45 mm的水平，位于孤立束核（NTS）和髓网状核的背侧（MdD）和腹侧（MdV）部分。NTS的免疫反应性细胞体比MdD和MdV多3倍，这些细胞体位于NTS尾侧三分之一的中线、内侧和外侧亚核。在GLP‐1和胰高血糖素抗血清或胰高血糖素和胰高血糖素抗血清孵育的成对相邻切片中，对同一细胞体进行免疫染色，提供了三种抗原在NTS同一神经元内共存的证据。对GLP - 1、胰高血糖素和glicentin抗血清具有免疫反应的神经纤维和神经末梢广泛分布在大鼠脑内，并且对这三种抗血清具有免疫反应的神经纤维和神经末梢的分布没有明显差异。免疫染色纤维和终末密度最高的是下丘脑、丘脑和间隔区，最低的是皮层和后脑。含有GLP - 1、胰高血糖素和glicentin的神经元细胞体在NTS、MdD和MdV内的定位，以及免疫反应纤维和终末在大鼠大脑中的广泛分布，表明这些肽在自主神经和中枢神经系统功能的整合中起着重要作用。
Although glucagonlike immunoreactants (GLIs) are present in the central nervous system of several mammalian species, their structural relationship with pancreatic proglucagon is not defined, and their precise anatomical distribution has not been studied extensively. To obtain further information about the structure and biological significance of brain GLIs, the anatomical distribution of three different antigenic determinants of pancreatic proglucagon‐‐glucagonlike peptide I (GLP‐I), glucagon, and glicentin‐‐was mapped in the brain of colchicine‐treated rats by immunocytochemistry using the avidin‐biotin‐peroxidase method.Neuronal cell bodies immunoreactive with antisera specific for GLP‐I, glucagon, and glicentin were found only in the caudal medulla oblongata. Within the caudal medulla immunostained cell bodies were found at levels from approximately 0.55 mm rostral to the obex to 0.45 mm caudal to the obex, and were located within the nucleus of the solitary tract (NTS) and the dorsal (MdD) and ventral (MdV) parts of the medullary reticular nucleus. The NTS contained three times more immunoreactive cell bodies than the MdD and MdV, and these cell bodies were located in the midline, medial, and lateral subnuclei of the caudal third of the NTS. Immunostaining of the same cell bodies in paired adjacent sections incubated with GLP‐I and glucagon antisera or glucagon and glicentin antisera provided evidence for coexistence of the three antigens within the same neurons of the NTS. Nerve fibers and terminals immunoreactive with GLP‐I, glucagon, and glicentin antisera were widely distributed throughout the rat brain and there was no discernible difference in the distribution of fibers and terminals immunoreactive with each of the three antisera. The highest densities of immunostained fibers and terminals were observed in the hypothalamus, thalamus, and septal regions, and the lowest in the cortex and hindbrain. The localization of neuronal cell bodies containing GLP‐I, glucagon, and glicentin within the NTS and the MdD and MdV, and the extensive distribution of immunoreactive fibers and terminals throughout the rat brain suggest a role for these peptides in the integration of autonomic as well as central nervous system functions.