STUDIES ON REPRESSION OF BETA-GALACTOSIDASE IN ESCHERICHIA COLI
STUDIES ON REPRESSION OF BETA-GALACTOSIDASE IN ESCHERICHIA COLI
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DOI：
10.1016/0926-6569(64)90272-x
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发表时间：
1964-01-01
期刊：
影响因子：
--
通讯作者：
MARR, AG
中科院分区：
文献类型：
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作者：
CLARK, DJ;MARR, AG
利用营养限制技术，在大肠杆菌突变株和产气气杆菌突变株JF-4中验证了分解代谢抑制的机制。已确定了两种不同类型的酶控制：一种是被诱导剂拮抗的对照，称为“诱导剂特异性抑制”；另一种是与诱导剂浓度无关的对照，称为“非诱导剂非依赖性抑制”。采用了一种分析方法，允许对这两种类型的压制进行定量测量。限制分解代谢活性导致去抑制，限制合成代谢活性导致抑制；然而，诱导剂专一性和非诱导剂非依赖性抑制的相对变化是不同的。碳限制导致诱导剂专一性抑制优先减少，而氮和硫限制导致诱导剂非依赖性抑制优先增加。
The mechanism of catabolite repression has been tested in mutants of E. coli and a mutant of Aerobacter aerogenes, JF-4, using the technic of nutrient limitation. Two distinct types of enzyme control have been identified: a control which is antagonized by inducer and is called "inducer-specific repression"; and a control which is independent of the concentration of inducer and is called "inducer-independent repression". A method of analysis is employed which allows each of the two types of repression to be measured quantitatively. The restriction of catabolic activities leads to derepression while the restriction of anabolic activities leads to repression; however, the relative change in inducer-specific and inducer-independent repression are not the same. Carbon limitation leads to a preferential decrease in inducer-specific repression while nitrogen and sulfur limitation lead to a preferential increase in inducer-independent repression.