Microbial hosts for metabolic engineering of lignin bioconversion to renewable chemicals
Microbial hosts for metabolic engineering of lignin bioconversion to renewable chemicals
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DOI：
10.1016/j.rser.2021.111674
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发表时间：
2021-10-06
影响因子：
15.9
通讯作者：
Alberti, Fabrizio
中科院分区：
文献类型：
--
作者：
Bugg, Timothy D. H.;Williamson, James J.;Alberti, Fabrizio
本文综述了利用工程微生物从植物生物质中生物转化木质素生产可再生化学品的研究进展。讨论了木质素生物转化的现有细菌宿主，如恶臭假单胞菌KT2440和约氏红球菌RHA1，并描述了它们被设计来产生芳香和非芳香产物的案例研究，以及用于这些研究的不同类型的木质素底物。对其他木质素降解菌进行了描述，并对利用其他细菌作为木质素代谢工程宿主的前景进行了讨论。讨论了真菌基因修饰的最新进展，为木质素降解真菌的代谢工程转化木质素生物产品提供了可能。随着该领域从实验室走向工业应用，讨论了该领域的前景和挑战，包括：底盘有机体的选择，木质素原料的选择，聚合木质素分解的复杂性，以及放大和选择生物制品的考虑。
This review discusses the use of engineered microbes for bioconversion of lignin from plant biomass to produce renewable chemicals. Existing bacterial hosts for lignin bioconversion are discussed, such as Pseudomonas putida KT2440 and Rhodococcus jostii RHA1, and case studies where they have been engineered to generate aromatic and non-aromatic products are described, and the different types of lignin substrates used for these studies. Other bacteria identified as lignin degraders are described, and the prospects for using other bacteria as hosts for metabolic engineering of lignin degradation are discussed. Recent advances in genetic modification of fungi are also discussed, which could lead to the metabolic engineering of lignin-degrading fungi for bioproduct formation from lignin. Prospects and challenges in this field are discussed, as the field moves from the laboratory to industrial application, including: choice of chassis organism, choice of lignin feedstock, the complexity of polymeric lignin breakdown, and considerations for scale-up and choice of bioproduct.