Enhanced expression of eotaxin and CCR3 in atopic dermatitis
Enhanced expression of eotaxin and CCR3 in atopic dermatitis
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DOI：
10.1046/j.1523-1747.1999.00619.x
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发表时间：
1999-07-01
影响因子：
6.5
通讯作者：
Baggiolini, M
中科院分区：
文献类型：
--
作者：
Yawalkar, N;Uguccioni, M;Baggiolini, M
趋化因子被认为在特应性皮炎的炎症发展中起重要作用。Eotaxin是一种CC趋化因子，是一种有效的化学引诱物和人嗜酸性粒细胞、嗜碱性粒细胞和Th 2淋巴细胞的激活剂，其通过趋化因子受体CCR 3起作用。我们通过免疫组化和巢式逆转录聚合酶链反应研究了Eotaxin和CCR 3以及MCP-3、MIP-1 α和白细胞介素-8在特应性皮炎和正常皮肤中的表达。皮肤活检标本取自异位性皮炎患者和非异位性对照组的非病变和病变皮肤。免疫反应性和转录的嗜酸性粒细胞趋化因子和CCR 3显着增加，在病变皮肤的特应性皮炎，但不是在非特应性对照。锌非损伤性特应性皮炎样品中CCR 3表达在mRNA和蛋白水平上也显著增加，而嗜酸性粒细胞趋化因子仅在mRNA水平上增加。在特应性皮炎和非特应性对照的皮肤样本中，MCP-3、MIP-1 α和白细胞介素-8的表达无显著差异。嗜酸性粒细胞活化趋化因子的增强的局部产生可能导致嗜酸性粒细胞和T淋巴细胞的募集，它们都表达CCR 3并有助于炎症的启动和维持。
Chemokines are thought to play an important part in the development of inflammation in atopic dermatitis. Eotaxin, a CC chemokine, is a potent chemoattractant and activator of human eosinophils, basophils and Th2 lymphocytes which acts via the chemokine receptor CCR3. We studied the expression of eotaxin and CCR3, as well as MCP-3, MIP-1 alpha and interleukin-8, in atopic dermatitis and normal skin by immunohistochemistry and nested reverse transcriptase-polymerase chain reaction. Skin biopsy specimens were obtained from nonlesional and lesional skin of patients with atopic dermatitis and of nonatopic controls. Immunoreactivity and transcripts of eotaxin and CCR3 were significantly increased in lesional skin from atopic dermatitis, but not in nonatopic controls. Zn nonlesional atopic dermatitis samples CCR3 expression was also significantly increased at the mRNA and protein level, whereas eotaxin was increased at the mRNA level only. No significant difference in the expression of MCP-3, MIP-1 alpha, and interleukin-8 was observed between skin samples from atopic dermatitis and nonatopic controls. The enhanced local production of eotaxin may lead to the recruitment of eosinophils and T lymphocytes, which both express CCR3 and contribute to the initiation and maintenance of inflammation.